Abstract
In the present study, we investigated the pharmacological characteristics of electrically stimulated [3H]-serotonin release from mammalian iris-ciliary bodies. Isolated bovine and human iris-ciliary bodies were loaded with [3H]-serotonin, superfused with Krebs buffer solution and then stimulated with trains of 300 direct current (d.c.) pulses to initiate the release of the transmitter. The modification of this [3H]-serotonin release process by various serotonergic agonists and antagonists was studied in order to define the pharmacology of serotonin receptor(s) present in the iris-ciliary body. In bovine iris-ciliary body, electrically-evoked [3H]-serotonin release was calcium-dependent, tetrodotoxin-sensitive and was enhanced by serotonin (EC50 = 200 nM) and 5-carboxmidotryptamine (EC50 = 4 nM). The rank order of potency of agonists in enhancing field-stimulated [3H]-serotonin release was: 5-carboamidotryptamine > m-chlorophenylbiguanide > 2-methyl-5-hydroxytryptamine = 5-methoxy-dimethyltryptamine > serotonin > 5-methoxy-tryptamine ≫ L-694,247 = α-methyl-5-hydroxytryptamine ≫ CGS 12066A = 8-hydroxy-2-(di-n-propylamino)tetraline. Serotonin and m-chlorophenylbiguanide also enhanced electrically-evoked [3H]-serotonin release from human iris-ciliary bodies with EC50s of 3 μM and 30 nM, respectively. The pharmacological profile displayed by serotonin receptor agonists was supported by the potent antagonism of the serotonin-induced enhancement of [3H]-serotonin release by 5HT7 receptor antagonists SB-258718 (IC50 = 18.6 ± 1.2 nM; n = 4) and mesulergine (IC50 = 0.26 ± 0.05 nM; n = 4). However, antagonists at 5HT6 and 5HT3 receptors exhibited a relatively weak blockade of serotonin induced enhancement of field-stimulated [3H]-serotonin release. These studies have shown the presence of functionally active prejunctional 5HT7 autoreceptors regulating the release of [3H]-serotonin from bovine iris-ciliary bodies, Excitatory prejunctional 5-HT autoreceptors also exist in human iris-ciliary bodies. It is possible that these serotonin autoreceptors may have relevance to the regulation of aqueous humor dynamics in the anterior uvea.
| Original language | English |
|---|---|
| Pages (from-to) | 59-67 |
| Number of pages | 9 |
| Journal | Experimental Eye Research |
| Volume | 73 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2001 |
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All Science Journal Classification (ASJC) codes
- Ophthalmology
- Sensory Systems
Cite this
[3H]-Serotonin release from bovine iris-ciliary body : Pharmacology of prejunctional serotonin (5-HT7) autoreceptors. / Harris, Lydia C.; Awe, S. Olubusayo; Opere, Catherine A.; LeDay, Angela M.; Ohia, Sunny E.; Sharif, Najam A.
In: Experimental Eye Research, Vol. 73, No. 1, 2001, p. 59-67.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - [3H]-Serotonin release from bovine iris-ciliary body
T2 - Pharmacology of prejunctional serotonin (5-HT7) autoreceptors
AU - Harris, Lydia C.
AU - Awe, S. Olubusayo
AU - Opere, Catherine A.
AU - LeDay, Angela M.
AU - Ohia, Sunny E.
AU - Sharif, Najam A.
PY - 2001
Y1 - 2001
N2 - In the present study, we investigated the pharmacological characteristics of electrically stimulated [3H]-serotonin release from mammalian iris-ciliary bodies. Isolated bovine and human iris-ciliary bodies were loaded with [3H]-serotonin, superfused with Krebs buffer solution and then stimulated with trains of 300 direct current (d.c.) pulses to initiate the release of the transmitter. The modification of this [3H]-serotonin release process by various serotonergic agonists and antagonists was studied in order to define the pharmacology of serotonin receptor(s) present in the iris-ciliary body. In bovine iris-ciliary body, electrically-evoked [3H]-serotonin release was calcium-dependent, tetrodotoxin-sensitive and was enhanced by serotonin (EC50 = 200 nM) and 5-carboxmidotryptamine (EC50 = 4 nM). The rank order of potency of agonists in enhancing field-stimulated [3H]-serotonin release was: 5-carboamidotryptamine > m-chlorophenylbiguanide > 2-methyl-5-hydroxytryptamine = 5-methoxy-dimethyltryptamine > serotonin > 5-methoxy-tryptamine ≫ L-694,247 = α-methyl-5-hydroxytryptamine ≫ CGS 12066A = 8-hydroxy-2-(di-n-propylamino)tetraline. Serotonin and m-chlorophenylbiguanide also enhanced electrically-evoked [3H]-serotonin release from human iris-ciliary bodies with EC50s of 3 μM and 30 nM, respectively. The pharmacological profile displayed by serotonin receptor agonists was supported by the potent antagonism of the serotonin-induced enhancement of [3H]-serotonin release by 5HT7 receptor antagonists SB-258718 (IC50 = 18.6 ± 1.2 nM; n = 4) and mesulergine (IC50 = 0.26 ± 0.05 nM; n = 4). However, antagonists at 5HT6 and 5HT3 receptors exhibited a relatively weak blockade of serotonin induced enhancement of field-stimulated [3H]-serotonin release. These studies have shown the presence of functionally active prejunctional 5HT7 autoreceptors regulating the release of [3H]-serotonin from bovine iris-ciliary bodies, Excitatory prejunctional 5-HT autoreceptors also exist in human iris-ciliary bodies. It is possible that these serotonin autoreceptors may have relevance to the regulation of aqueous humor dynamics in the anterior uvea.
AB - In the present study, we investigated the pharmacological characteristics of electrically stimulated [3H]-serotonin release from mammalian iris-ciliary bodies. Isolated bovine and human iris-ciliary bodies were loaded with [3H]-serotonin, superfused with Krebs buffer solution and then stimulated with trains of 300 direct current (d.c.) pulses to initiate the release of the transmitter. The modification of this [3H]-serotonin release process by various serotonergic agonists and antagonists was studied in order to define the pharmacology of serotonin receptor(s) present in the iris-ciliary body. In bovine iris-ciliary body, electrically-evoked [3H]-serotonin release was calcium-dependent, tetrodotoxin-sensitive and was enhanced by serotonin (EC50 = 200 nM) and 5-carboxmidotryptamine (EC50 = 4 nM). The rank order of potency of agonists in enhancing field-stimulated [3H]-serotonin release was: 5-carboamidotryptamine > m-chlorophenylbiguanide > 2-methyl-5-hydroxytryptamine = 5-methoxy-dimethyltryptamine > serotonin > 5-methoxy-tryptamine ≫ L-694,247 = α-methyl-5-hydroxytryptamine ≫ CGS 12066A = 8-hydroxy-2-(di-n-propylamino)tetraline. Serotonin and m-chlorophenylbiguanide also enhanced electrically-evoked [3H]-serotonin release from human iris-ciliary bodies with EC50s of 3 μM and 30 nM, respectively. The pharmacological profile displayed by serotonin receptor agonists was supported by the potent antagonism of the serotonin-induced enhancement of [3H]-serotonin release by 5HT7 receptor antagonists SB-258718 (IC50 = 18.6 ± 1.2 nM; n = 4) and mesulergine (IC50 = 0.26 ± 0.05 nM; n = 4). However, antagonists at 5HT6 and 5HT3 receptors exhibited a relatively weak blockade of serotonin induced enhancement of field-stimulated [3H]-serotonin release. These studies have shown the presence of functionally active prejunctional 5HT7 autoreceptors regulating the release of [3H]-serotonin from bovine iris-ciliary bodies, Excitatory prejunctional 5-HT autoreceptors also exist in human iris-ciliary bodies. It is possible that these serotonin autoreceptors may have relevance to the regulation of aqueous humor dynamics in the anterior uvea.
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U2 - 10.1006/exer.2001.1012
DO - 10.1006/exer.2001.1012
M3 - Article
VL - 73
SP - 59
EP - 67
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 1
ER -