Surface-modified solid lipid nanoparticulate formulation for ifosfamide: development and characterization.

Ambrish A. Pandit, Alekha K. Dash

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present research focuses on the development of the surface modified solid lipid nanoparticulate (SLN) system for enhancing the stability and sustaining the release of a model hydrophilic drug ifosfamide. SLNs consisting of glyceryl monooleate (GMO) and chitosan were prepared by double emulsion technique, crosslinked with sodium tripolyphosphate, followed by lyophilization under two different vacuum conditions. The physicochemical characterization of SLNs included evaluation of surface morphology, particle size and surface charge, moisture content and physical state of the drug in the delivery system. The in vitro drug release and the stability were evaluated using high-performance liquid chromatography and liquid chromatography/mass spectrometry, respectively. Cellular permeability and subcellular localization studies were performed using Caco-2 cells. Different chamber pressures during lyophilization produced SLNs with different morphologies and moisture contents. SLNs demonstrated high encapsulation efficiency, sustained release, and enhanced stability of ifosfamide with a high cellular uptake and permeability for Caco-2 cells. GMO and chitosan SLNs could be successfully used for enhancing the stability, sustaining the release, enhancing the targeting and permeability characteristics of ifosfamide.

Original languageEnglish
Pages (from-to)1397-1412
Number of pages16
JournalNanomedicine
Volume6
Issue number8
StatePublished - Oct 2011

Fingerprint

Ifosfamide
Lipids
Permeability
Caco-2 Cells
Freeze Drying
Chitosan
Drug Stability
Moisture
Pharmaceutical Preparations
Drug Delivery Systems
Vacuum
Emulsions
Particle Size
Liquid Chromatography
Mass Spectrometry
Liquid chromatography
High performance liquid chromatography
Surface charge
High Pressure Liquid Chromatography
Encapsulation

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Surface-modified solid lipid nanoparticulate formulation for ifosfamide : development and characterization. / Pandit, Ambrish A.; Dash, Alekha K.

In: Nanomedicine, Vol. 6, No. 8, 10.2011, p. 1397-1412.

Research output: Contribution to journalArticle

@article{cd85106b96694f778b8352efb84fdda7,
title = "Surface-modified solid lipid nanoparticulate formulation for ifosfamide: development and characterization.",
abstract = "The present research focuses on the development of the surface modified solid lipid nanoparticulate (SLN) system for enhancing the stability and sustaining the release of a model hydrophilic drug ifosfamide. SLNs consisting of glyceryl monooleate (GMO) and chitosan were prepared by double emulsion technique, crosslinked with sodium tripolyphosphate, followed by lyophilization under two different vacuum conditions. The physicochemical characterization of SLNs included evaluation of surface morphology, particle size and surface charge, moisture content and physical state of the drug in the delivery system. The in vitro drug release and the stability were evaluated using high-performance liquid chromatography and liquid chromatography/mass spectrometry, respectively. Cellular permeability and subcellular localization studies were performed using Caco-2 cells. Different chamber pressures during lyophilization produced SLNs with different morphologies and moisture contents. SLNs demonstrated high encapsulation efficiency, sustained release, and enhanced stability of ifosfamide with a high cellular uptake and permeability for Caco-2 cells. GMO and chitosan SLNs could be successfully used for enhancing the stability, sustaining the release, enhancing the targeting and permeability characteristics of ifosfamide.",
author = "Pandit, {Ambrish A.} and Dash, {Alekha K.}",
year = "2011",
month = "10",
language = "English",
volume = "6",
pages = "1397--1412",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "Future Medicine Ltd.",
number = "8",

}

TY - JOUR

T1 - Surface-modified solid lipid nanoparticulate formulation for ifosfamide

T2 - development and characterization.

AU - Pandit, Ambrish A.

AU - Dash, Alekha K.

PY - 2011/10

Y1 - 2011/10

N2 - The present research focuses on the development of the surface modified solid lipid nanoparticulate (SLN) system for enhancing the stability and sustaining the release of a model hydrophilic drug ifosfamide. SLNs consisting of glyceryl monooleate (GMO) and chitosan were prepared by double emulsion technique, crosslinked with sodium tripolyphosphate, followed by lyophilization under two different vacuum conditions. The physicochemical characterization of SLNs included evaluation of surface morphology, particle size and surface charge, moisture content and physical state of the drug in the delivery system. The in vitro drug release and the stability were evaluated using high-performance liquid chromatography and liquid chromatography/mass spectrometry, respectively. Cellular permeability and subcellular localization studies were performed using Caco-2 cells. Different chamber pressures during lyophilization produced SLNs with different morphologies and moisture contents. SLNs demonstrated high encapsulation efficiency, sustained release, and enhanced stability of ifosfamide with a high cellular uptake and permeability for Caco-2 cells. GMO and chitosan SLNs could be successfully used for enhancing the stability, sustaining the release, enhancing the targeting and permeability characteristics of ifosfamide.

AB - The present research focuses on the development of the surface modified solid lipid nanoparticulate (SLN) system for enhancing the stability and sustaining the release of a model hydrophilic drug ifosfamide. SLNs consisting of glyceryl monooleate (GMO) and chitosan were prepared by double emulsion technique, crosslinked with sodium tripolyphosphate, followed by lyophilization under two different vacuum conditions. The physicochemical characterization of SLNs included evaluation of surface morphology, particle size and surface charge, moisture content and physical state of the drug in the delivery system. The in vitro drug release and the stability were evaluated using high-performance liquid chromatography and liquid chromatography/mass spectrometry, respectively. Cellular permeability and subcellular localization studies were performed using Caco-2 cells. Different chamber pressures during lyophilization produced SLNs with different morphologies and moisture contents. SLNs demonstrated high encapsulation efficiency, sustained release, and enhanced stability of ifosfamide with a high cellular uptake and permeability for Caco-2 cells. GMO and chitosan SLNs could be successfully used for enhancing the stability, sustaining the release, enhancing the targeting and permeability characteristics of ifosfamide.

UR - http://www.scopus.com/inward/record.url?scp=84861154736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861154736&partnerID=8YFLogxK

M3 - Article

C2 - 22091968

AN - SCOPUS:84861154736

VL - 6

SP - 1397

EP - 1412

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 8

ER -