Surgical treatment of liver injury with microwave tissue coagulation

An experimental study

Akira Takasu, Hirofumi Norio, Toshihisa Sakamoto, Yoshiaki Okada, Palmer Q. Bessey, Juan A. Asensio, Vernon Henderson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: The purpose of this study was to examine whether microwave tissue coagulation (MTC) therapy is capable of stopping bleeding from severe liver injury in pigs. Methods: Ten pigs (38 ± 4 kg) underwent a 30-mL/kg isovolemic exchange transfusion with 3% low-molecular-weight dextran to produce dilutional coagulopathy, and then a through-and-through laceration injury measuring approximately 8 cm in length was induced in the right hepatic lobe. Immediately after inflicting the injury, the animals were randomly divided into two groups: Group A (n = 5, MTC was repeated along the liver laceration at intervals of 2.0 cm with manual compression) or Group B (n = 5, the injured lobe was manually compressed without MTC therapy for 1 minute). All animals received lactated Ringer's solution to maintain the mean arterial pressure at 75 mm Hg for 1 hour after the abdominal closure. The intraperitoneal blood loss, mean arterial pressure, volume of lactated Ringer's solution, and hematologic variables were compared between the groups. For further laboratory evaluation, three additional experimental animals were treated with the MTC therapy after inflicting the injury and then were allowed to survive for 14 days. Results: Mean arterial pressure declined from a mean value of 88 ± 10 mm Hg (range, 75-107 mm Hg) to 62 ± 3 mm Hg (range, 50-75 mm Hg) after the induction of liver injury. The total blood loss in Group A was 192 ± 58 g (range, 120-250 g), which was lower (p > 0.01) than that of 448 ±138 g (range, 260-650 g) in Group B. The resuscitation fluid volume of Group A animals was 304 ± 204 mL (range, 100-600 mL), which was smaller (p <0.01) than that of 1,320 ± 654 mL (range, 900-2,250 mL) in Group B. At 14 days, all three animals that were treated in the additional study were found to be in good health. Their necropsies showed no evidence of an intrahepatic abscess, hematoma, or biloma. Conclusion: MTC therapy was thus found to provide simple, rapid, and definitive hemorrhage control in cases of severe liver injury without die need for reoperation.

Original languageEnglish
Pages (from-to)984-990
Number of pages7
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume56
Issue number5
StatePublished - May 2004

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Microwaves
Cell- and Tissue-Based Therapy
Liver
Wounds and Injuries
Arterial Pressure
Lacerations
Swine
Therapeutics
Hemorrhage
Dextrans
Reoperation
Resuscitation
Hematoma
Abscess
Molecular Weight
Health

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Surgical treatment of liver injury with microwave tissue coagulation : An experimental study. / Takasu, Akira; Norio, Hirofumi; Sakamoto, Toshihisa; Okada, Yoshiaki; Bessey, Palmer Q.; Asensio, Juan A.; Henderson, Vernon.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 56, No. 5, 05.2004, p. 984-990.

Research output: Contribution to journalArticle

Takasu, A, Norio, H, Sakamoto, T, Okada, Y, Bessey, PQ, Asensio, JA & Henderson, V 2004, 'Surgical treatment of liver injury with microwave tissue coagulation: An experimental study', Journal of Trauma - Injury, Infection and Critical Care, vol. 56, no. 5, pp. 984-990.
Takasu, Akira ; Norio, Hirofumi ; Sakamoto, Toshihisa ; Okada, Yoshiaki ; Bessey, Palmer Q. ; Asensio, Juan A. ; Henderson, Vernon. / Surgical treatment of liver injury with microwave tissue coagulation : An experimental study. In: Journal of Trauma - Injury, Infection and Critical Care. 2004 ; Vol. 56, No. 5. pp. 984-990.
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