SV40 T Antigen Directed by a Powerful Erythroid Enhancer–Promoter Produced Sarcomas and Pancreatic Tumors But Not Erythroid-Specific Tumors in Transgenic Mice

Tal Teitz, T. s.Benedict Yen, Judy C. Chang, Yuet Wai Kan

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have expressed the simian virus 40 (SV40) large T antigen oncogene in erythroid tissues of mice to test its ability to immortilize erythroid cells. A transgene construct was built in which the SV40 large T antigen structural gene was linked to erythroid-specific enhancer and promoter sequences. The enhancer employed was the human β-globin family microlocus control region, and the promoter sequences were derived from the human β-globin promoter. Transgenic mice were generated and they expressed T antigen in the bone marrow and spleen cells. Yet, no hematopoietic neoplasia arose in these mice. Instead, after a lag period of 2–6 months, the mice developed soft tissue sarcomas and pancreatic islet-cell tumors that expressed high levels of T antigen.

Original languageEnglish (US)
Pages (from-to)705-710
Number of pages6
JournalDNA and Cell Biology
Volume13
Issue number7
DOIs
StatePublished - Jul 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

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