Abstract
Molecular inversion probes (MIPs) in combination with massively parallel DNA sequencing represent a versatile, yet economical tool for targeted sequencing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput sequencing at relatively low cost per sample. Here, we describe a “wet bench” protocol detailing the capture and subsequent sequencing of >2000 genomic targets from 192 samples, representative of a single lane on the Illumina HiSeq 2000 platform.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 95-106 |
| Number of pages | 12 |
| Volume | 1492 |
| DOIs | |
| State | Published - Jan 1 2017 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1492 |
| ISSN (Print) | 1064-3745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). / Cantsilieris, Stuart; Stessman, Holly; Shendure, Jay; Eichler, Evan E.
Methods in Molecular Biology. Vol. 1492 Humana Press Inc., 2017. p. 95-106 (Methods in Molecular Biology; Vol. 1492).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs)
AU - Cantsilieris, Stuart
AU - Stessman, Holly
AU - Shendure, Jay
AU - Eichler, Evan E.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Molecular inversion probes (MIPs) in combination with massively parallel DNA sequencing represent a versatile, yet economical tool for targeted sequencing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput sequencing at relatively low cost per sample. Here, we describe a “wet bench” protocol detailing the capture and subsequent sequencing of >2000 genomic targets from 192 samples, representative of a single lane on the Illumina HiSeq 2000 platform.
AB - Molecular inversion probes (MIPs) in combination with massively parallel DNA sequencing represent a versatile, yet economical tool for targeted sequencing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput sequencing at relatively low cost per sample. Here, we describe a “wet bench” protocol detailing the capture and subsequent sequencing of >2000 genomic targets from 192 samples, representative of a single lane on the Illumina HiSeq 2000 platform.
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UR - http://www.scopus.com/inward/citedby.url?scp=84994860454&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6442-0_6
DO - 10.1007/978-1-4939-6442-0_6
M3 - Chapter
C2 - 27822858
AN - SCOPUS:84994860454
VL - 1492
T3 - Methods in Molecular Biology
SP - 95
EP - 106
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -