Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs)

Stuart Cantsilieris, Holly Stessman, Jay Shendure, Evan E. Eichler

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Molecular inversion probes (MIPs) in combination with massively parallel DNA sequencing represent a versatile, yet economical tool for targeted sequencing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput sequencing at relatively low cost per sample. Here, we describe a “wet bench” protocol detailing the capture and subsequent sequencing of >2000 genomic targets from 192 samples, representative of a single lane on the Illumina HiSeq 2000 platform.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-106
Number of pages12
Volume1492
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1492
ISSN (Print)1064-3745

Fingerprint

Molecular Probes
DNA Sequence Analysis
High-Throughput Nucleotide Sequencing
Oligonucleotides
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Cantsilieris, S., Stessman, H., Shendure, J., & Eichler, E. E. (2017). Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). In Methods in Molecular Biology (Vol. 1492, pp. 95-106). (Methods in Molecular Biology; Vol. 1492). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6442-0_6

Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). / Cantsilieris, Stuart; Stessman, Holly; Shendure, Jay; Eichler, Evan E.

Methods in Molecular Biology. Vol. 1492 Humana Press Inc., 2017. p. 95-106 (Methods in Molecular Biology; Vol. 1492).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cantsilieris, S, Stessman, H, Shendure, J & Eichler, EE 2017, Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). in Methods in Molecular Biology. vol. 1492, Methods in Molecular Biology, vol. 1492, Humana Press Inc., pp. 95-106. https://doi.org/10.1007/978-1-4939-6442-0_6
Cantsilieris S, Stessman H, Shendure J, Eichler EE. Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). In Methods in Molecular Biology. Vol. 1492. Humana Press Inc. 2017. p. 95-106. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6442-0_6
Cantsilieris, Stuart ; Stessman, Holly ; Shendure, Jay ; Eichler, Evan E. / Targeted capture and high-throughput sequencing using molecular inversion probes (MIPs). Methods in Molecular Biology. Vol. 1492 Humana Press Inc., 2017. pp. 95-106 (Methods in Molecular Biology).
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