Abstract
Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.
| Original language | English |
|---|---|
| Pages (from-to) | 230-234 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 269 |
| Issue number | 5221 |
| State | Published - 1995 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- General
Cite this
Targeted disruption of mouse EGF receptor : Effect of genetic background on mutant phenotype. / Threadgill, David W.; Dlugosz, Andrzej A.; Hansen, Laura A.; Tennenbaum, Tamar; Lichti, Ulrike; Yee, Della; LaMantia, Christian; Mourton, Tracy; Herrup, Karl; Harris, Raymond C.; Barnard, John A.; Yuspa, Stuart H.; Coffey, Robert J.; Magnuson, Terry.
In: Science, Vol. 269, No. 5221, 1995, p. 230-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeted disruption of mouse EGF receptor
T2 - Effect of genetic background on mutant phenotype
AU - Threadgill, David W.
AU - Dlugosz, Andrzej A.
AU - Hansen, Laura A.
AU - Tennenbaum, Tamar
AU - Lichti, Ulrike
AU - Yee, Della
AU - LaMantia, Christian
AU - Mourton, Tracy
AU - Herrup, Karl
AU - Harris, Raymond C.
AU - Barnard, John A.
AU - Yuspa, Stuart H.
AU - Coffey, Robert J.
AU - Magnuson, Terry
PY - 1995
Y1 - 1995
N2 - Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.
AB - Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.
UR - http://www.scopus.com/inward/record.url?scp=0029064203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029064203&partnerID=8YFLogxK
M3 - Article
VL - 269
SP - 230
EP - 234
JO - Science
JF - Science
SN - 0036-8075
IS - 5221
ER -