Targeted disruption of mouse EGF receptor: Effect of genetic background on mutant phenotype

David W. Threadgill; Andrzej A. Dlugosz; Laura A. Hansen; Tamar Tennenbaum; Ulrike Lichti; Della Yee; Christian LaMantia; Tracy Mourton; Karl Herrup; Raymond C. Harris; John A. Barnard; Stuart H. Yuspa; Robert J. Coffey; Terry Magnuson

doi:10.1126/science.7618084

Targeted disruption of mouse EGF receptor: Effect of genetic background on mutant phenotype

David W. Threadgill, Andrzej A. Dlugosz, Laura A. Hansen, Tamar Tennenbaum, Ulrike Lichti, Della Yee, Christian LaMantia, Tracy Mourton, Karl Herrup, Raymond C. Harris, John A. Barnard, Stuart H. Yuspa, Robert J. Coffey, Terry Magnuson

Research output: Contribution to journal › Article

1182 Scopus citations

Abstract

Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.

Original language	English (US)
Pages (from-to)	230-234
Number of pages	5
Journal	Science
Volume	269
Issue number	5221
DOIs	https://doi.org/10.1126/science.7618084
State	Published - Jan 1 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

General

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Threadgill, D. W., Dlugosz, A. A., Hansen, L. A., Tennenbaum, T., Lichti, U., Yee, D., LaMantia, C., Mourton, T., Herrup, K., Harris, R. C., Barnard, J. A., Yuspa, S. H., Coffey, R. J., & Magnuson, T. (1995). Targeted disruption of mouse EGF receptor: Effect of genetic background on mutant phenotype. Science, 269(5221), 230-234. https://doi.org/10.1126/science.7618084

Targeted disruption of mouse EGF receptor : Effect of genetic background on mutant phenotype. / Threadgill, David W.; Dlugosz, Andrzej A.; Hansen, Laura A.; Tennenbaum, Tamar; Lichti, Ulrike; Yee, Della; LaMantia, Christian; Mourton, Tracy; Herrup, Karl; Harris, Raymond C.; Barnard, John A.; Yuspa, Stuart H.; Coffey, Robert J.; Magnuson, Terry.

In: Science, Vol. 269, No. 5221, 01.01.1995, p. 230-234.

Research output: Contribution to journal › Article

Threadgill, David W. ; Dlugosz, Andrzej A. ; Hansen, Laura A. ; Tennenbaum, Tamar ; Lichti, Ulrike ; Yee, Della ; LaMantia, Christian ; Mourton, Tracy ; Herrup, Karl ; Harris, Raymond C. ; Barnard, John A. ; Yuspa, Stuart H. ; Coffey, Robert J. ; Magnuson, Terry. / Targeted disruption of mouse EGF receptor : Effect of genetic background on mutant phenotype. In: Science. 1995 ; Vol. 269, No. 5221. pp. 230-234.

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abstract = "Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.",

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