The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: Detection in a complex β-lactamase background

Mark D. Reisbig; Nancy D. Hanson

The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: Detection in a complex β-lactamase background

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Abstract

The purpose of this study was to identify the genetic organization and inducibility of bla_ACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the bla_ACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in bla_ACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.

Original language	English (US)
Pages (from-to)	557-560
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	49
Issue number	3
State	Published - Mar 25 2002

All Science Journal Classification (ASJC) codes

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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Reisbig, M. D., & Hanson, N. D. (2002). The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: Detection in a complex β-lactamase background. Journal of Antimicrobial Chemotherapy, 49(3), 557-560.

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