The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: Detection in a complex β-lactamase background

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Abstract

The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.

Original languageEnglish
Pages (from-to)557-560
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume49
Issue number3
StatePublished - 2002

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Enterobacter cloacae
Cefoxitin
Klebsiella pneumoniae
Plasmids
RNA
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Pharmacology

Cite this

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abstract = "The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.",
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T1 - The ACT-1 plasmid-encoded AmpC β-lactamase is inducible

T2 - Detection in a complex β-lactamase background

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AU - Hanson, Nancy D.

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N2 - The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.

AB - The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.

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