Abstract
The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.
| Original language | English |
|---|---|
| Pages (from-to) | 557-560 |
| Number of pages | 4 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 49 |
| Issue number | 3 |
| State | Published - 2002 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Pharmacology
Cite this
The ACT-1 plasmid-encoded AmpC β-lactamase is inducible : Detection in a complex β-lactamase background. / Reisbig, Mark; Hanson, Nancy D.
In: Journal of Antimicrobial Chemotherapy, Vol. 49, No. 3, 2002, p. 557-560.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ACT-1 plasmid-encoded AmpC β-lactamase is inducible
T2 - Detection in a complex β-lactamase background
AU - Reisbig, Mark
AU - Hanson, Nancy D.
PY - 2002
Y1 - 2002
N2 - The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.
AB - The purpose of this study was to identify the genetic organization and inducibility of blaACT-1 in a clinical isolate of Klebsiella pneumoniae possessing at least five different β-lactamases. The genetic organization of the blaACT-1lampR region is identical to those of inducible chromosomal ampC genes. RNA analysis using primer extension demonstrated a five-fold increase in blaACT-1 transcript production on exposure to cefoxitin. These findings are significant because induction was detected in a complicated β-lactamase background. In addition, this report is the first to describe an inducible plasmid-encoded AmpC β-lactamase of Enterobacter cloacae origin.
UR - http://www.scopus.com/inward/record.url?scp=0036118955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036118955&partnerID=8YFLogxK
M3 - Article
VL - 49
SP - 557
EP - 560
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 3
ER -