The bounty of RAGs: Recombination signal complexes and reaction outcomes

Research output: Contribution to journalReview article

78 Citations (Scopus)

Abstract

V(D)J recombination is a form of site-specific DNA rearrangement through which antigen receptor genes are assembled. This process involves the breakage and reunion of DNA mediated by two lymphoid cell-specific proteins, recombination activating genes RAG-1 and RAG-2, and ubiquitously expressed architectural DNA-binding proteins and DNA-repair factors. Here I review the progress toward understanding the composition, assembly, organization, and activity of the protein-DNA complexes that support the initiation of V(D)J recombination, as well as the molecular basis for the sequence-specific recognition of recombination signal sequences (RSSs) that are the targets of the RAG proteins. Parallels are drawn between V(D)J recombination and Tn5/Tn10 transposition with respect to the reactions, the proteins, and the protein-DNA complexes involved in these processes. I also consider the relative roles of the different sequence elements within the RSS in recognition, cleavage, and post-cleavage events. Finally, I discuss alternative DNA transactions mediated by the V(D)J recombinase, the protein-DNA complexes that support them, and factors and forces that control them.

Original languageEnglish
Pages (from-to)90-114
Number of pages25
JournalImmunological Reviews
Volume200
DOIs
StatePublished - Aug 2004

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Genetic Recombination
V(D)J Recombination
DNA
Proteins
Protein Sorting Signals
VDJ Recombinases
RAG-1 Genes
Reunion
Antigen Receptors
Gene Rearrangement
DNA-Binding Proteins
DNA Repair
Lymphocytes
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

The bounty of RAGs : Recombination signal complexes and reaction outcomes. / Swanson, Patrick.

In: Immunological Reviews, Vol. 200, 08.2004, p. 90-114.

Research output: Contribution to journalReview article

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