The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation

Jinglin L. Xie, Longguang Qin, Zhengqiang Miao, Ben T. Grys, Jacinto De La Cruz Diaz, Kenneth Ting, Jonathan R. Krieger, Jiefei Tong, Kaeling Tan, Michelle D. Leach, Troy Ketela, Michael F. Moran, Damian J. Krysan, Charles Boone, Brenda J. Andrews, Anna Selmecki, Koon Ho Wong, Nicole Robbins, Leah E. Cowen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The capacity to coordinate environmental sensing with initiation of cellular responses underpins microbial survival and is crucial for virulence and stress responses in microbial pathogens. Here we define circuitry that enables the fungal pathogen Candida albicans to couple cell cycle dynamics with responses to cell wall stress induced by echinocandins, a front-line class of antifungal drugs. We discover that the C. albicans transcription factor Cas5 is crucial for proper cell cycle dynamics and responses to echinocandins, which inhibit β-1,3-glucan synthesis. Cas5 has distinct transcriptional targets under basal and stress conditions, is activated by the phosphatase Glc7, and can regulate the expression of target genes in concert with the transcriptional regulators Swi4 and Swi6. Thus, we illuminate a mechanism of transcriptional control that couples cell wall integrity with cell cycle regulation, and uncover circuitry governing antifungal drug resistance.

Original languageEnglish (US)
Article number499
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Candida
Candida albicans
Drug Resistance
Echinocandins
Cell Cycle
drugs
Transcription Factors
Cells
Cell Wall
cycles
Fungal Drug Resistance
Pharmaceutical Preparations
pathogens
Pathogens
Glucans
Phosphoric Monoester Hydrolases
Virulence
virulence
phosphatases
regulators

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation. / Xie, Jinglin L.; Qin, Longguang; Miao, Zhengqiang; Grys, Ben T.; Diaz, Jacinto De La Cruz; Ting, Kenneth; Krieger, Jonathan R.; Tong, Jiefei; Tan, Kaeling; Leach, Michelle D.; Ketela, Troy; Moran, Michael F.; Krysan, Damian J.; Boone, Charles; Andrews, Brenda J.; Selmecki, Anna; Ho Wong, Koon; Robbins, Nicole; Cowen, Leah E.

In: Nature Communications, Vol. 8, No. 1, 499, 01.12.2017.

Research output: Contribution to journalArticle

Xie, JL, Qin, L, Miao, Z, Grys, BT, Diaz, JDLC, Ting, K, Krieger, JR, Tong, J, Tan, K, Leach, MD, Ketela, T, Moran, MF, Krysan, DJ, Boone, C, Andrews, BJ, Selmecki, A, Ho Wong, K, Robbins, N & Cowen, LE 2017, 'The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation', Nature Communications, vol. 8, no. 1, 499. https://doi.org/10.1038/s41467-017-00547-y
Xie, Jinglin L. ; Qin, Longguang ; Miao, Zhengqiang ; Grys, Ben T. ; Diaz, Jacinto De La Cruz ; Ting, Kenneth ; Krieger, Jonathan R. ; Tong, Jiefei ; Tan, Kaeling ; Leach, Michelle D. ; Ketela, Troy ; Moran, Michael F. ; Krysan, Damian J. ; Boone, Charles ; Andrews, Brenda J. ; Selmecki, Anna ; Ho Wong, Koon ; Robbins, Nicole ; Cowen, Leah E. / The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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