The conserved tetrameric subunit stoichiometry of Slc26 proteins

Richard J. Hallworth, Kelsey Stark, Lyandysha Zholudeva, Benjamin B. Currall, Michael G. Nichols

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The Slc26 family proteins, with one possible exception, transport anions across membranes in a wide variety of tissues in vertebrates, invertebrates, and plants. Mutations in human members of the family are a significant cause of disease. Slc26 family proteins are thought to be oligomers, but their stoichiometry of association is in dispute. A recent study, using sequential bleaching of single fluorophore-coupled molecules in membrane fragments, demonstrated that mammalian Slc26a5 (prestin) is a tetramer. In this article, the stoichiometry of two nonmammalian prestins and three human SLC26 proteins has been analyzed by the same method, including the evolutionarily-distant SLC26A11. The analysis showed that tetramerization is common and likely to be ubiquitous among Slc26 proteins, at least in vertebrates. The implication of the findings is that tetramerization is present for functional reasons.

Original languageEnglish
Pages (from-to)799-807
Number of pages9
JournalMicroscopy and Microanalysis
Volume19
Issue number4
DOIs
StatePublished - Aug 2013

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Stoichiometry
stoichiometry
proteins
Proteins
vertebrates
invertebrates
membranes
Membranes
Fluorophores
bleaching
mutations
Bleaching
oligomers
Oligomers
Negative ions
fragments
Association reactions
Tissue
anions
Molecules

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

The conserved tetrameric subunit stoichiometry of Slc26 proteins. / Hallworth, Richard J.; Stark, Kelsey; Zholudeva, Lyandysha; Currall, Benjamin B.; Nichols, Michael G.

In: Microscopy and Microanalysis, Vol. 19, No. 4, 08.2013, p. 799-807.

Research output: Contribution to journalArticle

Hallworth, Richard J. ; Stark, Kelsey ; Zholudeva, Lyandysha ; Currall, Benjamin B. ; Nichols, Michael G. / The conserved tetrameric subunit stoichiometry of Slc26 proteins. In: Microscopy and Microanalysis. 2013 ; Vol. 19, No. 4. pp. 799-807.
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