The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41

Janos Sumegi, Ji Yi Wang, Dong Kai Zhen, James D. Eudy, Catherine B. Talmadge, Bi Fang Li, Peter Berglund, Michael Weston, Su Fang Yao, Manling Ma-Edmonds, Larry Overbeck, Philip M. Kelley, Eugene Zabarovsky, Eva Uzvolgyi, Eric J. Stanbridge, George Klein, William J. Kimberling

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Abstract

The gene for Usher syndrome type II (USH2A), an autosomal recessive syndromic deafness, has been mapped to a region of 1q41 flanked proximally by D1S217 and distally by D1S439. Using sequence-tagged sites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated and ordered into a single contig that spans approximately 11.0 Mb. The order of microsatellite and STS markers in this region was established as D1S505- D1S425-DXS217-D1S556-D1S237-D1S474-EB1-EB2-KB6-AFM144XF2-KB1-K B4-D1S229- D1S490-D1S227-TGFβ2-D1S439. Analysis of newly positioned polymorphic markers in recombinant individuals in two Usher syndrome type IIa families has enabled us to identify DXS474 and AFM144XF2 as two flanking markers for the Usher type IIa locus. The physical distance between the two markers is 1.0 Mb. This region is covered by eight YACs from the CEPH library: 945f7, 867g9, 762a6, 919h3, 794b8, 785h4, 848b9, and 841g2. A long-range physical map of the Usher type IIa critical region, using MluI, BssHII, NotI, EagI, and SacII, has been developed.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalGenomics
Volume35
Issue number1
DOIs
StatePublished - Jul 1 1996

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Usher Syndromes
Sequence Tagged Sites
Yeast Artificial Chromosomes
Microsatellite Repeats
Genes
Libraries
Clone Cells

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Sumegi, J., Wang, J. Y., Zhen, D. K., Eudy, J. D., Talmadge, C. B., Li, B. F., ... Kimberling, W. J. (1996). The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41. Genomics, 35(1), 79-86. https://doi.org/10.1006/geno.1996.0325

The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41. / Sumegi, Janos; Wang, Ji Yi; Zhen, Dong Kai; Eudy, James D.; Talmadge, Catherine B.; Li, Bi Fang; Berglund, Peter; Weston, Michael; Yao, Su Fang; Ma-Edmonds, Manling; Overbeck, Larry; Kelley, Philip M.; Zabarovsky, Eugene; Uzvolgyi, Eva; Stanbridge, Eric J.; Klein, George; Kimberling, William J.

In: Genomics, Vol. 35, No. 1, 01.07.1996, p. 79-86.

Research output: Contribution to journalArticle

Sumegi, J, Wang, JY, Zhen, DK, Eudy, JD, Talmadge, CB, Li, BF, Berglund, P, Weston, M, Yao, SF, Ma-Edmonds, M, Overbeck, L, Kelley, PM, Zabarovsky, E, Uzvolgyi, E, Stanbridge, EJ, Klein, G & Kimberling, WJ 1996, 'The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41', Genomics, vol. 35, no. 1, pp. 79-86. https://doi.org/10.1006/geno.1996.0325
Sumegi, Janos ; Wang, Ji Yi ; Zhen, Dong Kai ; Eudy, James D. ; Talmadge, Catherine B. ; Li, Bi Fang ; Berglund, Peter ; Weston, Michael ; Yao, Su Fang ; Ma-Edmonds, Manling ; Overbeck, Larry ; Kelley, Philip M. ; Zabarovsky, Eugene ; Uzvolgyi, Eva ; Stanbridge, Eric J. ; Klein, George ; Kimberling, William J. / The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41. In: Genomics. 1996 ; Vol. 35, No. 1. pp. 79-86.
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abstract = "The gene for Usher syndrome type II (USH2A), an autosomal recessive syndromic deafness, has been mapped to a region of 1q41 flanked proximally by D1S217 and distally by D1S439. Using sequence-tagged sites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated and ordered into a single contig that spans approximately 11.0 Mb. The order of microsatellite and STS markers in this region was established as D1S505- D1S425-DXS217-D1S556-D1S237-D1S474-EB1-EB2-KB6-AFM144XF2-KB1-K B4-D1S229- D1S490-D1S227-TGFβ2-D1S439. Analysis of newly positioned polymorphic markers in recombinant individuals in two Usher syndrome type IIa families has enabled us to identify DXS474 and AFM144XF2 as two flanking markers for the Usher type IIa locus. The physical distance between the two markers is 1.0 Mb. This region is covered by eight YACs from the CEPH library: 945f7, 867g9, 762a6, 919h3, 794b8, 785h4, 848b9, and 841g2. A long-range physical map of the Usher type IIa critical region, using MluI, BssHII, NotI, EagI, and SacII, has been developed.",
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AU - Berglund, Peter

AU - Weston, Michael

AU - Yao, Su Fang

AU - Ma-Edmonds, Manling

AU - Overbeck, Larry

AU - Kelley, Philip M.

AU - Zabarovsky, Eugene

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AU - Klein, George

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