Abstract
The gene for Usher syndrome type II (USH2A), an autosomal recessive syndromic deafness, has been mapped to a region of 1q41 flanked proximally by D1S217 and distally by D1S439. Using sequence-tagged sites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated and ordered into a single contig that spans approximately 11.0 Mb. The order of microsatellite and STS markers in this region was established as D1S505- D1S425-DXS217-D1S556-D1S237-D1S474-EB1-EB2-KB6-AFM144XF2-KB1-K B4-D1S229- D1S490-D1S227-TGFβ2-D1S439. Analysis of newly positioned polymorphic markers in recombinant individuals in two Usher syndrome type IIa families has enabled us to identify DXS474 and AFM144XF2 as two flanking markers for the Usher type IIa locus. The physical distance between the two markers is 1.0 Mb. This region is covered by eight YACs from the CEPH library: 945f7, 867g9, 762a6, 919h3, 794b8, 785h4, 848b9, and 841g2. A long-range physical map of the Usher type IIa critical region, using MluI, BssHII, NotI, EagI, and SacII, has been developed.
| Original language | English |
|---|---|
| Pages (from-to) | 79-86 |
| Number of pages | 8 |
| Journal | Genomics |
| Volume | 35 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 1996 |
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All Science Journal Classification (ASJC) codes
- Genetics
Cite this
The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41. / Sumegi, Janos; Wang, Ji Yi; Zhen, Dong Kai; Eudy, James D.; Talmadge, Catherine B.; Li, Bi Fang; Berglund, Peter; Weston, Michael; Yao, Su Fang; Ma-Edmonds, Manling; Overbeck, Larry; Kelley, Philip M.; Zabarovsky, Eugene; Uzvolgyi, Eva; Stanbridge, Eric J.; Klein, George; Kimberling, William J.
In: Genomics, Vol. 35, No. 1, 01.07.1996, p. 79-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41
AU - Sumegi, Janos
AU - Wang, Ji Yi
AU - Zhen, Dong Kai
AU - Eudy, James D.
AU - Talmadge, Catherine B.
AU - Li, Bi Fang
AU - Berglund, Peter
AU - Weston, Michael
AU - Yao, Su Fang
AU - Ma-Edmonds, Manling
AU - Overbeck, Larry
AU - Kelley, Philip M.
AU - Zabarovsky, Eugene
AU - Uzvolgyi, Eva
AU - Stanbridge, Eric J.
AU - Klein, George
AU - Kimberling, William J.
PY - 1996/7/1
Y1 - 1996/7/1
N2 - The gene for Usher syndrome type II (USH2A), an autosomal recessive syndromic deafness, has been mapped to a region of 1q41 flanked proximally by D1S217 and distally by D1S439. Using sequence-tagged sites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated and ordered into a single contig that spans approximately 11.0 Mb. The order of microsatellite and STS markers in this region was established as D1S505- D1S425-DXS217-D1S556-D1S237-D1S474-EB1-EB2-KB6-AFM144XF2-KB1-K B4-D1S229- D1S490-D1S227-TGFβ2-D1S439. Analysis of newly positioned polymorphic markers in recombinant individuals in two Usher syndrome type IIa families has enabled us to identify DXS474 and AFM144XF2 as two flanking markers for the Usher type IIa locus. The physical distance between the two markers is 1.0 Mb. This region is covered by eight YACs from the CEPH library: 945f7, 867g9, 762a6, 919h3, 794b8, 785h4, 848b9, and 841g2. A long-range physical map of the Usher type IIa critical region, using MluI, BssHII, NotI, EagI, and SacII, has been developed.
AB - The gene for Usher syndrome type II (USH2A), an autosomal recessive syndromic deafness, has been mapped to a region of 1q41 flanked proximally by D1S217 and distally by D1S439. Using sequence-tagged sites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated and ordered into a single contig that spans approximately 11.0 Mb. The order of microsatellite and STS markers in this region was established as D1S505- D1S425-DXS217-D1S556-D1S237-D1S474-EB1-EB2-KB6-AFM144XF2-KB1-K B4-D1S229- D1S490-D1S227-TGFβ2-D1S439. Analysis of newly positioned polymorphic markers in recombinant individuals in two Usher syndrome type IIa families has enabled us to identify DXS474 and AFM144XF2 as two flanking markers for the Usher type IIa locus. The physical distance between the two markers is 1.0 Mb. This region is covered by eight YACs from the CEPH library: 945f7, 867g9, 762a6, 919h3, 794b8, 785h4, 848b9, and 841g2. A long-range physical map of the Usher type IIa critical region, using MluI, BssHII, NotI, EagI, and SacII, has been developed.
UR - http://www.scopus.com/inward/record.url?scp=0030201074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030201074&partnerID=8YFLogxK
U2 - 10.1006/geno.1996.0325
DO - 10.1006/geno.1996.0325
M3 - Article
C2 - 8661107
AN - SCOPUS:0030201074
VL - 35
SP - 79
EP - 86
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -