The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions

D. F. Levine, D. E. Witherspoon, J. L. Gutmann, Martha E. Nunn, J. T. Newman, A. M. Iacopino

Research output: Contribution to journalArticle

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Abstract

Aim: The purpose of this study was to elucidate whether a decrease/increase in T-cell populations is present in the development of periradicular disease in the immuno-compromised feline model. Methodology: Eight cats were immunosuppressed with steroids prior to infection with feline immunodeficiency virus (FIV). Another eight cats, age and sex matched littermates, were monitored and tested at equivalent periods of time and served as uninoculated, seronegative controls. Periradicular lesions were induced using local bacterial inoculations into the pulp of the canine teeth and assessed after one- and four-week periods, corresponding to the acute and chronic stages of the periradicular disease. Block sections were obtained and specimens were prepared for H & E and immunohistochemical staining for CD4+ and CD8+ receptors. Cells were quantified using a computer imaging system and data analysed using generalized estimating equation (GEE) regression models. Results: Significantly lower CD4+ counts and CD4+/ CD8+ ratios were observed at all time periods in the periradicularregion of the FIV group (P=0.0006). No significant difference in CD8+ counts was observed between the two groups. In both groups there was a significant difference in the CD4+ counts between one week and baseline, and 1 week and 4 weeks. There was no significant difference between baseline and 4 weeks for either group. Conclusion: FIV infection reflected decreased CD4+ counts at the periradicular level, however, inflammation and progression of the lesion, appeared to be comparable to the non-immunocompromised controls.

Original languageEnglish
Pages (from-to)586-593
Number of pages8
JournalInternational Endodontic Journal
Volume34
Issue number8
StatePublished - Dec 2001
Externally publishedYes

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Feline Immunodeficiency Virus
Virus Diseases
CD4 Lymphocyte Count
Cats
Cuspid
CD4 Antigens
CD4-CD8 Ratio
Felidae
Computer Systems
Steroids
Staining and Labeling
Inflammation
T-Lymphocytes
Infection
Population

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Levine, D. F., Witherspoon, D. E., Gutmann, J. L., Nunn, M. E., Newman, J. T., & Iacopino, A. M. (2001). The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions. International Endodontic Journal, 34(8), 586-593.

The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions. / Levine, D. F.; Witherspoon, D. E.; Gutmann, J. L.; Nunn, Martha E.; Newman, J. T.; Iacopino, A. M.

In: International Endodontic Journal, Vol. 34, No. 8, 12.2001, p. 586-593.

Research output: Contribution to journalArticle

Levine, DF, Witherspoon, DE, Gutmann, JL, Nunn, ME, Newman, JT & Iacopino, AM 2001, 'The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions', International Endodontic Journal, vol. 34, no. 8, pp. 586-593.
Levine DF, Witherspoon DE, Gutmann JL, Nunn ME, Newman JT, Iacopino AM. The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions. International Endodontic Journal. 2001 Dec;34(8):586-593.
Levine, D. F. ; Witherspoon, D. E. ; Gutmann, J. L. ; Nunn, Martha E. ; Newman, J. T. ; Iacopino, A. M. / The effect of FIV infection on CD4+ and CD8+ counts in periradicular lesions. In: International Endodontic Journal. 2001 ; Vol. 34, No. 8. pp. 586-593.
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