The effect of pertussis and beta adrenergic-blocking agents on mast cells

Helmy M. Guirgis, Robert G. Townley

Research output: Contribution to journalArticle

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Abstract

Pertussis vaccine injected ip in doses known to cause hypersensitization resulted in a marked decrease in the number of mast cells recovered from the peritoneal washings of rats and mice. A significant reduction was obtained as early as one day after pertussis injection of ten billion cells in rats and was marked after 5 to 7 days. A maximum reduction in the number of mast cells was obtained by a dose of 20 billion cells. There was no detectable histamine biological activity in the supernatant from peritoneal washings obtained after 10 min, 60 min, and 24 hr from control and pertussis-treated rats, indicating that pertussis did not cause degranulation of mast cells in vivo. The histamine content in the precipitated mast cell pellets from control rats was much higher than the corresponding histamine content from pertussis-treated rats. In rats and mice, propranolol and other beta adrenergic-blocking agents caused degranulation of mast cells in the peritoneal washings in vitro. Practolol was the least effective beta adrenergic-blocking agent in degranulating mast cells. Catecholamines, histamine, 6-hydroxydopamine, methacholine, and pertussis failed to cause any degranulation. Isoproterenol protected the mast cell against the degranulation induced by propranolol. Propranolol caused bluing in rat and mice skin when injected id. Mast cells from control and pertussis-injected rats were equally sensitive to propranolol in vitro. The low recovery of mast cells from the peritoneal washings of rats and mice is thought to be due to mobilization of mast cells away from the peritoneum.

Original languageEnglish
Pages (from-to)241-249
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume58
Issue number2
DOIs
StatePublished - 1976

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Adrenergic beta-Antagonists
Whooping Cough
Mast Cells
Propranolol
Histamine
Practolol
Pertussis Vaccine
Cell Degranulation
Methacholine Chloride
Oxidopamine
Peritoneum
Isoproterenol
Catecholamines
Skin
Injections

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

Cite this

The effect of pertussis and beta adrenergic-blocking agents on mast cells. / Guirgis, Helmy M.; Townley, Robert G.

In: Journal of Allergy and Clinical Immunology, Vol. 58, No. 2, 1976, p. 241-249.

Research output: Contribution to journalArticle

Guirgis, Helmy M. ; Townley, Robert G. / The effect of pertussis and beta adrenergic-blocking agents on mast cells. In: Journal of Allergy and Clinical Immunology. 1976 ; Vol. 58, No. 2. pp. 241-249.
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