The EGFR is required for proper innervation to the skin

Adel Maklad, Jodi R. Nicolai, Kyle J. Bichsel, Jackie E. Evenson, Tang Cheng Lee, David W. Threadgill, Laura A. Hansen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

EGFR family members are essential for proper peripheral nervous system development. A role for EGFR itself in peripheral nervous system development in vivo, however, has not been reported. We investigated whether EGFR is required for cutaneous innervation using Egfr null and skin-targeted Egfr mutant mice. Neuronal markers; including PGP9.5, GAP-43, acetylated tubulin, and neurofilaments; revealed that Egfr null dorsal skin was hyperinnervated with a disorganized pattern of innervation. In addition, receptor subtypes such as lanceolate endings were disorganized and immature. To determine whether the hyperinnervation phenotype resulted from a target-derived effect of loss of EGFR, mice lacking EGFR expression in the cutaneous epithelium were examined. These mice retained other aspects of the cutaneous Egfr null phenotype but exhibited normal innervation. The sensory deficits in Egfr null dorsal skin were not associated with any abnormality in the morphology or density of dorsal root ganglion (DRG) neurons or Schwann cells. However, explant and dissociated cell cultures of DRG revealed more extensive branching in Egfr null cultures. These data demonstrate that EGFR is required for proper cutaneous innervation during development and suggest that it limits axonal outgrowth and branching in a DRG-autonomous manner.

Original languageEnglish
Pages (from-to)690-698
Number of pages9
JournalJournal of Investigative Dermatology
Volume129
Issue number3
DOIs
StatePublished - Mar 2009

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Skin
Neurology
Spinal Ganglia
GAP-43 Protein
Tubulin
Cell culture
Peripheral Nervous System
Neurons
Cells
Phenotype
Intermediate Filaments
Schwann Cells
Epithelium
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Maklad, A., Nicolai, J. R., Bichsel, K. J., Evenson, J. E., Lee, T. C., Threadgill, D. W., & Hansen, L. A. (2009). The EGFR is required for proper innervation to the skin. Journal of Investigative Dermatology, 129(3), 690-698. https://doi.org/10.1038/jid.2008.281

The EGFR is required for proper innervation to the skin. / Maklad, Adel; Nicolai, Jodi R.; Bichsel, Kyle J.; Evenson, Jackie E.; Lee, Tang Cheng; Threadgill, David W.; Hansen, Laura A.

In: Journal of Investigative Dermatology, Vol. 129, No. 3, 03.2009, p. 690-698.

Research output: Contribution to journalArticle

Maklad, A, Nicolai, JR, Bichsel, KJ, Evenson, JE, Lee, TC, Threadgill, DW & Hansen, LA 2009, 'The EGFR is required for proper innervation to the skin', Journal of Investigative Dermatology, vol. 129, no. 3, pp. 690-698. https://doi.org/10.1038/jid.2008.281
Maklad A, Nicolai JR, Bichsel KJ, Evenson JE, Lee TC, Threadgill DW et al. The EGFR is required for proper innervation to the skin. Journal of Investigative Dermatology. 2009 Mar;129(3):690-698. https://doi.org/10.1038/jid.2008.281
Maklad, Adel ; Nicolai, Jodi R. ; Bichsel, Kyle J. ; Evenson, Jackie E. ; Lee, Tang Cheng ; Threadgill, David W. ; Hansen, Laura A. / The EGFR is required for proper innervation to the skin. In: Journal of Investigative Dermatology. 2009 ; Vol. 129, No. 3. pp. 690-698.
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