The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice

Mark A. Rainey, Manju George, Guoguang Ying, Reiko Akakura, Daniel J. Burgess, Ed Siefker, Tom Bargar, Lynn Doglio, Susan E. Crawford, Gordon L. Todd, Venkatesh Govindarajan, Rex A. Hess, Vimla Band, Mayumi Naramura, Hamid Band

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background. The C-terminal Eps15 homology domain-containing protein 1 (EHD1) is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1 -/- mice using a Cre/loxP system. Results. Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1 -/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions. Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.

Original languageEnglish
Article number37
JournalBMC Developmental Biology
Volume10
DOIs
StatePublished - 2010

Fingerprint

Spermatogenesis
Spermatids
Fertility
Seminiferous Tubules
Testis
Seminiferous Epithelium
Acrosome
Sertoli Cells
Epididymis
Phagocytosis
In Situ Hybridization
Spermatozoa
Electron Microscopy
Immunohistochemistry
Protein Domains
Parturition
Light
Membranes

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

Rainey, M. A., George, M., Ying, G., Akakura, R., Burgess, D. J., Siefker, E., ... Band, H. (2010). The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice. BMC Developmental Biology, 10, [37]. https://doi.org/10.1186/1471-213X-10-37

The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice. / Rainey, Mark A.; George, Manju; Ying, Guoguang; Akakura, Reiko; Burgess, Daniel J.; Siefker, Ed; Bargar, Tom; Doglio, Lynn; Crawford, Susan E.; Todd, Gordon L.; Govindarajan, Venkatesh; Hess, Rex A.; Band, Vimla; Naramura, Mayumi; Band, Hamid.

In: BMC Developmental Biology, Vol. 10, 37, 2010.

Research output: Contribution to journalArticle

Rainey, MA, George, M, Ying, G, Akakura, R, Burgess, DJ, Siefker, E, Bargar, T, Doglio, L, Crawford, SE, Todd, GL, Govindarajan, V, Hess, RA, Band, V, Naramura, M & Band, H 2010, 'The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice', BMC Developmental Biology, vol. 10, 37. https://doi.org/10.1186/1471-213X-10-37
Rainey, Mark A. ; George, Manju ; Ying, Guoguang ; Akakura, Reiko ; Burgess, Daniel J. ; Siefker, Ed ; Bargar, Tom ; Doglio, Lynn ; Crawford, Susan E. ; Todd, Gordon L. ; Govindarajan, Venkatesh ; Hess, Rex A. ; Band, Vimla ; Naramura, Mayumi ; Band, Hamid. / The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice. In: BMC Developmental Biology. 2010 ; Vol. 10.
@article{a9972b68ec79492db4e1af14b41bdbdd,
title = "The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice",
abstract = "Background. The C-terminal Eps15 homology domain-containing protein 1 (EHD1) is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1 -/- mice using a Cre/loxP system. Results. Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1 -/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions. Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.",
author = "Rainey, {Mark A.} and Manju George and Guoguang Ying and Reiko Akakura and Burgess, {Daniel J.} and Ed Siefker and Tom Bargar and Lynn Doglio and Crawford, {Susan E.} and Todd, {Gordon L.} and Venkatesh Govindarajan and Hess, {Rex A.} and Vimla Band and Mayumi Naramura and Hamid Band",
year = "2010",
doi = "10.1186/1471-213X-10-37",
language = "English",
volume = "10",
journal = "BMC Developmental Biology",
issn = "1471-213X",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice

AU - Rainey, Mark A.

AU - George, Manju

AU - Ying, Guoguang

AU - Akakura, Reiko

AU - Burgess, Daniel J.

AU - Siefker, Ed

AU - Bargar, Tom

AU - Doglio, Lynn

AU - Crawford, Susan E.

AU - Todd, Gordon L.

AU - Govindarajan, Venkatesh

AU - Hess, Rex A.

AU - Band, Vimla

AU - Naramura, Mayumi

AU - Band, Hamid

PY - 2010

Y1 - 2010

N2 - Background. The C-terminal Eps15 homology domain-containing protein 1 (EHD1) is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1 -/- mice using a Cre/loxP system. Results. Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1 -/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions. Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.

AB - Background. The C-terminal Eps15 homology domain-containing protein 1 (EHD1) is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1 -/- mice using a Cre/loxP system. Results. Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1 -/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions. Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.

UR - http://www.scopus.com/inward/record.url?scp=77950529317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950529317&partnerID=8YFLogxK

U2 - 10.1186/1471-213X-10-37

DO - 10.1186/1471-213X-10-37

M3 - Article

VL - 10

JO - BMC Developmental Biology

JF - BMC Developmental Biology

SN - 1471-213X

M1 - 37

ER -