The endocytic recycling regulatory protein EHD1 Is required for ocular lens development

Priyanka Arya, Mark A. Rainey, Sohinee Bhattacharyya, Bhopal C. Mohapatra, Manju George, Murali R. Kuracha, Matthew D. Storck, Vimla Band, Venkatesh Govindarajan, Hamid Band

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The C-terminal Eps15 homology domain-containing (EHD) proteins play a key role in endocytic recycling, a fundamental cellular process that ensures the return of endocytosed membrane components and receptors back to the cell surface. To define the. in vivo biological functions of EHD1, we have generated. Ehd1 knockout mice and previously reported a requirement of EHD1 for spermatogenesis. Here, we show that approximately 56% of the. Ehd1-null mice displayed gross ocular abnormalities, including anophthalmia, aphakia, microphthalmia and congenital cataracts. Histological characterization of ocular abnormalities showed pleiotropic defects that include a smaller or absent lens, persistence of lens stalk and hyaloid vasculature, and deformed optic cups. To test whether these profound ocular defects resulted from the loss of EHD1 in the lens or in non-lenticular tissues, we deleted the. Ehd1 gene selectively in the presumptive lens ectoderm using. Le-Cre. Conditional. Ehd1 deletion in the lens resulted in developmental defects that included thin epithelial layers, small lenses and absence of corneal endothelium + Ehd1 deletion in the lens also resulted in reduced lens epithelial proliferation, survival and expression of junctional proteins E-cadherin and ZO-1. Finally,. Le-Cre-mediated deletion of. Ehd1 in the lens led to defects in corneal endothelial differentiation. Taken together, these data reveal a unique role for EHD1 in early lens development and suggest a previously unknown link between the endocytic recycling pathway and regulation of key developmental processes including proliferation, differentiation and morphogenesis.

Original languageEnglish
JournalDevelopmental Biology
DOIs
StateAccepted/In press - Mar 17 2015

Fingerprint

Crystalline Lens
Lenses
Proteins
Eye Abnormalities
Cadherins
Anophthalmos
Aphakia
Microphthalmos
Corneal Endothelium
Ectoderm
Spermatogenesis
Endocytosis
Morphogenesis
Knockout Mice
Cataract

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Arya, P., Rainey, M. A., Bhattacharyya, S., Mohapatra, B. C., George, M., Kuracha, M. R., ... Band, H. (Accepted/In press). The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. Developmental Biology. https://doi.org/10.1016/j.ydbio.2015.10.005

The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. / Arya, Priyanka; Rainey, Mark A.; Bhattacharyya, Sohinee; Mohapatra, Bhopal C.; George, Manju; Kuracha, Murali R.; Storck, Matthew D.; Band, Vimla; Govindarajan, Venkatesh; Band, Hamid.

In: Developmental Biology, 17.03.2015.

Research output: Contribution to journalArticle

Arya, P, Rainey, MA, Bhattacharyya, S, Mohapatra, BC, George, M, Kuracha, MR, Storck, MD, Band, V, Govindarajan, V & Band, H 2015, 'The endocytic recycling regulatory protein EHD1 Is required for ocular lens development', Developmental Biology. https://doi.org/10.1016/j.ydbio.2015.10.005
Arya, Priyanka ; Rainey, Mark A. ; Bhattacharyya, Sohinee ; Mohapatra, Bhopal C. ; George, Manju ; Kuracha, Murali R. ; Storck, Matthew D. ; Band, Vimla ; Govindarajan, Venkatesh ; Band, Hamid. / The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. In: Developmental Biology. 2015.
@article{564183087cba49d8acc7dbbf2ea6e51e,
title = "The endocytic recycling regulatory protein EHD1 Is required for ocular lens development",
abstract = "The C-terminal Eps15 homology domain-containing (EHD) proteins play a key role in endocytic recycling, a fundamental cellular process that ensures the return of endocytosed membrane components and receptors back to the cell surface. To define the. in vivo biological functions of EHD1, we have generated. Ehd1 knockout mice and previously reported a requirement of EHD1 for spermatogenesis. Here, we show that approximately 56{\%} of the. Ehd1-null mice displayed gross ocular abnormalities, including anophthalmia, aphakia, microphthalmia and congenital cataracts. Histological characterization of ocular abnormalities showed pleiotropic defects that include a smaller or absent lens, persistence of lens stalk and hyaloid vasculature, and deformed optic cups. To test whether these profound ocular defects resulted from the loss of EHD1 in the lens or in non-lenticular tissues, we deleted the. Ehd1 gene selectively in the presumptive lens ectoderm using. Le-Cre. Conditional. Ehd1 deletion in the lens resulted in developmental defects that included thin epithelial layers, small lenses and absence of corneal endothelium + Ehd1 deletion in the lens also resulted in reduced lens epithelial proliferation, survival and expression of junctional proteins E-cadherin and ZO-1. Finally,. Le-Cre-mediated deletion of. Ehd1 in the lens led to defects in corneal endothelial differentiation. Taken together, these data reveal a unique role for EHD1 in early lens development and suggest a previously unknown link between the endocytic recycling pathway and regulation of key developmental processes including proliferation, differentiation and morphogenesis.",
author = "Priyanka Arya and Rainey, {Mark A.} and Sohinee Bhattacharyya and Mohapatra, {Bhopal C.} and Manju George and Kuracha, {Murali R.} and Storck, {Matthew D.} and Vimla Band and Venkatesh Govindarajan and Hamid Band",
year = "2015",
month = "3",
day = "17",
doi = "10.1016/j.ydbio.2015.10.005",
language = "English",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - The endocytic recycling regulatory protein EHD1 Is required for ocular lens development

AU - Arya, Priyanka

AU - Rainey, Mark A.

AU - Bhattacharyya, Sohinee

AU - Mohapatra, Bhopal C.

AU - George, Manju

AU - Kuracha, Murali R.

AU - Storck, Matthew D.

AU - Band, Vimla

AU - Govindarajan, Venkatesh

AU - Band, Hamid

PY - 2015/3/17

Y1 - 2015/3/17

N2 - The C-terminal Eps15 homology domain-containing (EHD) proteins play a key role in endocytic recycling, a fundamental cellular process that ensures the return of endocytosed membrane components and receptors back to the cell surface. To define the. in vivo biological functions of EHD1, we have generated. Ehd1 knockout mice and previously reported a requirement of EHD1 for spermatogenesis. Here, we show that approximately 56% of the. Ehd1-null mice displayed gross ocular abnormalities, including anophthalmia, aphakia, microphthalmia and congenital cataracts. Histological characterization of ocular abnormalities showed pleiotropic defects that include a smaller or absent lens, persistence of lens stalk and hyaloid vasculature, and deformed optic cups. To test whether these profound ocular defects resulted from the loss of EHD1 in the lens or in non-lenticular tissues, we deleted the. Ehd1 gene selectively in the presumptive lens ectoderm using. Le-Cre. Conditional. Ehd1 deletion in the lens resulted in developmental defects that included thin epithelial layers, small lenses and absence of corneal endothelium + Ehd1 deletion in the lens also resulted in reduced lens epithelial proliferation, survival and expression of junctional proteins E-cadherin and ZO-1. Finally,. Le-Cre-mediated deletion of. Ehd1 in the lens led to defects in corneal endothelial differentiation. Taken together, these data reveal a unique role for EHD1 in early lens development and suggest a previously unknown link between the endocytic recycling pathway and regulation of key developmental processes including proliferation, differentiation and morphogenesis.

AB - The C-terminal Eps15 homology domain-containing (EHD) proteins play a key role in endocytic recycling, a fundamental cellular process that ensures the return of endocytosed membrane components and receptors back to the cell surface. To define the. in vivo biological functions of EHD1, we have generated. Ehd1 knockout mice and previously reported a requirement of EHD1 for spermatogenesis. Here, we show that approximately 56% of the. Ehd1-null mice displayed gross ocular abnormalities, including anophthalmia, aphakia, microphthalmia and congenital cataracts. Histological characterization of ocular abnormalities showed pleiotropic defects that include a smaller or absent lens, persistence of lens stalk and hyaloid vasculature, and deformed optic cups. To test whether these profound ocular defects resulted from the loss of EHD1 in the lens or in non-lenticular tissues, we deleted the. Ehd1 gene selectively in the presumptive lens ectoderm using. Le-Cre. Conditional. Ehd1 deletion in the lens resulted in developmental defects that included thin epithelial layers, small lenses and absence of corneal endothelium + Ehd1 deletion in the lens also resulted in reduced lens epithelial proliferation, survival and expression of junctional proteins E-cadherin and ZO-1. Finally,. Le-Cre-mediated deletion of. Ehd1 in the lens led to defects in corneal endothelial differentiation. Taken together, these data reveal a unique role for EHD1 in early lens development and suggest a previously unknown link between the endocytic recycling pathway and regulation of key developmental processes including proliferation, differentiation and morphogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84951735730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951735730&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2015.10.005

DO - 10.1016/j.ydbio.2015.10.005

M3 - Article

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

ER -