TY - JOUR
T1 - The Fat-Dachsous signaling pathway regulates growth of horns in Trypoxylus dichotomus, but does not affect horn allometry
AU - Hust, James
AU - Lavine, Mark D.
AU - Worthington, Amy M.
AU - Zinna, Robert
AU - Gotoh, Hiroki
AU - Niimi, T.
AU - Lavine, Laura
N1 - Funding Information:
This research was funded by the National Science Foundation IOS 0919730 (LL) and the National Institute of Food and Agriculture , U.S. Department of Agriculture, Hatch Project 1001738 (LL). HG was supported by a Grant for Basic Science Research from the Sumitomo Foundation and by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. RZ was supported by an NIH PERT fellowship, ID# 2K12GM000708-16. TN was supported by MEXT KAKENHI (16H01452). Appendix A
Funding Information:
This research was funded by the National Science Foundation IOS 0919730 (LL) and the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project 1001738 (LL). HG was supported by a Grant for Basic Science Research from the Sumitomo Foundation and by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. RZ was supported by an NIH PERT fellowship, ID# 2K12GM000708-16. TN was supported by MEXT KAKENHI (16H01452).
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/2
Y1 - 2018/2
N2 - Males of the Asian rhinoceros beetle, Trypoxylus dichotomus, possess exaggerated head and thoracic horns that scale dramatically out of proportion to body size. While studies of insulin signaling suggest that this pathway regulates nutrition-dependent growth including exaggerated horns, what regulates disproportionate growth has yet to be identified. The Fat signaling pathway is a potential candidate for regulating disproportionate growth of sexually-selected traits, a hypothesis we advanced in a previous paper (Gotoh et al., 2015). To investigate the role of Fat signaling in the growth and scaling of the sexually dimorphic, condition-dependent traits of the in the Asian rhinoceros beetle T. dichotomus, we used RNA interference to knock down expression of fat and its co-receptor dachsous. Knockdown of fat, and to a lesser degree dachsous, caused shortening and widening of appendages, including the head and thoracic horns. However, scaling of horns to body size was not affected. Our results show that Fat signaling regulates horn growth in T. dichotomus as it does in appendage growth in other insects. However, we provide evidence that Fat signaling does not mediate the disproportionate, positive allometric growth of horns in T. dichotomus.
AB - Males of the Asian rhinoceros beetle, Trypoxylus dichotomus, possess exaggerated head and thoracic horns that scale dramatically out of proportion to body size. While studies of insulin signaling suggest that this pathway regulates nutrition-dependent growth including exaggerated horns, what regulates disproportionate growth has yet to be identified. The Fat signaling pathway is a potential candidate for regulating disproportionate growth of sexually-selected traits, a hypothesis we advanced in a previous paper (Gotoh et al., 2015). To investigate the role of Fat signaling in the growth and scaling of the sexually dimorphic, condition-dependent traits of the in the Asian rhinoceros beetle T. dichotomus, we used RNA interference to knock down expression of fat and its co-receptor dachsous. Knockdown of fat, and to a lesser degree dachsous, caused shortening and widening of appendages, including the head and thoracic horns. However, scaling of horns to body size was not affected. Our results show that Fat signaling regulates horn growth in T. dichotomus as it does in appendage growth in other insects. However, we provide evidence that Fat signaling does not mediate the disproportionate, positive allometric growth of horns in T. dichotomus.
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U2 - 10.1016/j.jinsphys.2018.01.006
DO - 10.1016/j.jinsphys.2018.01.006
M3 - Article
C2 - 29366850
AN - SCOPUS:85041421790
VL - 105
SP - 85
EP - 94
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
ER -