The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

M. C. Lin, Y. Salomon, M. Rendell, M. Rodbell

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

Original languageEnglish (US)
Pages (from-to)4246-4252
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number11
StatePublished - Dec 1 1975

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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