The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

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Abstract

The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

Original languageEnglish
Pages (from-to)4246-4252
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number11
Publication statusPublished - 1975
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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