The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

M. C. Lin, Y. Salomon, M. Rendell, M. Rodbell

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

Original languageEnglish
Pages (from-to)4246-4252
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number11
StatePublished - 1975
Externally publishedYes

Fingerprint

Adenylyl Cyclases
Magnesium
Adenosine Triphosphate
Adenylyl Imidodiphosphate
Ions
Liver
Substrates
Glucagon
Application programs
Kinetics

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH. / Lin, M. C.; Salomon, Y.; Rendell, M.; Rodbell, M.

In: Journal of Biological Chemistry, Vol. 250, No. 11, 1975, p. 4246-4252.

Research output: Contribution to journalArticle

Lin, M. C. ; Salomon, Y. ; Rendell, M. ; Rodbell, M. / The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH. In: Journal of Biological Chemistry. 1975 ; Vol. 250, No. 11. pp. 4246-4252.
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