The interaction of cholera toxin subunit A with cultured adrenal cells

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Abstract

Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isolated 125I-labeled subunits A and B, peptides A1 and A2 and bovine serum albumin (BSA) by cultured adrenal cells was investigated. The results indicate that iodosubunit A, or peptide A1 or A2, traverses the plasma membrane and is released to the cell cytosol. A significant portion of bound iodosubunits A or B was associated with the plasma membrane, suggesting presence of specific membrane receptors. The biological activity of subunit A was determined by the mouse adrenal cell assay. The purified subunit caused a characteristic change from epithelioid to rounded morphology. A 30-fold higher concentration of subunit A (on a mole/mole basis) as compared with native toxin was required for a maximum morphologic response. These results extend previous observations related to the bioactivity of subunit A of the cholera enterotoxin molecule.

Original languageEnglish (US)
Pages (from-to)915-921
Number of pages7
JournalCanadian Journal of Microbiology
Volume24
Issue number8
DOIs
StatePublished - Jan 1 1978

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Cholera Toxin
Cultured Cells
formic acid
varespladib methyl
Cell Membrane
Disc Electrophoresis
Bovine Serum Albumin
Cytosol
Chromatography
Polyacrylamide Gel Electrophoresis
Gels
Peptides
Membranes
Vibrio cholerae stN protein

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

The interaction of cholera toxin subunit A with cultured adrenal cells. / Knoop, Floyd C.

In: Canadian Journal of Microbiology, Vol. 24, No. 8, 01.01.1978, p. 915-921.

Research output: Contribution to journalArticle

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