Abstract
Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p <0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p <0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p <0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.
| Original language | English |
|---|---|
| Pages (from-to) | 576-580 |
| Number of pages | 5 |
| Journal | Annals of Neurology |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2004 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Cite this
The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity. / Sullivan, Patrick G.; Rippy, Nancy A.; Simeone, Kristina A.; Concepcion, Rachele C.; Agarwal, Aakash K.; Rho, Jong M.
In: Annals of Neurology, Vol. 55, No. 4, 04.2004, p. 576-580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity
AU - Sullivan, Patrick G.
AU - Rippy, Nancy A.
AU - Simeone, Kristina A.
AU - Concepcion, Rachele C.
AU - Agarwal, Aakash K.
AU - Rho, Jong M.
PY - 2004/4
Y1 - 2004/4
N2 - Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p <0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p <0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p <0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.
AB - Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p <0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p <0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p <0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.
UR - http://www.scopus.com/inward/record.url?scp=1642289587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642289587&partnerID=8YFLogxK
U2 - 10.1002/ana.20062
DO - 10.1002/ana.20062
M3 - Article
C2 - 15048898
AN - SCOPUS:1642289587
VL - 55
SP - 576
EP - 580
JO - Annals of Neurology
JF - Annals of Neurology
SN - 0364-5134
IS - 4
ER -