The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity

Patrick G. Sullivan, Nancy A. Rippy, Kristina A. Simeone, Rachele C. Concepcion, Aakash K. Agarwal, Jong M. Rho

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p <0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p <0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p <0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.

Original languageEnglish
Pages (from-to)576-580
Number of pages5
JournalAnnals of Neurology
Volume55
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

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Ketogenic Diet
Reactive Oxygen Species
Diet
Oligomycins
Dentate Gyrus
Neuroprotective Agents
Respiratory Rate
Protons
Hippocampus
Protein Isoforms
Fatty Acids
Western Blotting
Uncoupling Protein 1
Mitochondrial Uncoupling Proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Sullivan, P. G., Rippy, N. A., Simeone, K. A., Concepcion, R. C., Agarwal, A. K., & Rho, J. M. (2004). The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity. Annals of Neurology, 55(4), 576-580. https://doi.org/10.1002/ana.20062

The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity. / Sullivan, Patrick G.; Rippy, Nancy A.; Simeone, Kristina A.; Concepcion, Rachele C.; Agarwal, Aakash K.; Rho, Jong M.

In: Annals of Neurology, Vol. 55, No. 4, 04.2004, p. 576-580.

Research output: Contribution to journalArticle

Sullivan, PG, Rippy, NA, Simeone, KA, Concepcion, RC, Agarwal, AK & Rho, JM 2004, 'The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity', Annals of Neurology, vol. 55, no. 4, pp. 576-580. https://doi.org/10.1002/ana.20062
Sullivan, Patrick G. ; Rippy, Nancy A. ; Simeone, Kristina A. ; Concepcion, Rachele C. ; Agarwal, Aakash K. ; Rho, Jong M. / The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity. In: Annals of Neurology. 2004 ; Vol. 55, No. 4. pp. 576-580.
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