The latency-related gene of bovine herpesvirus 1 inhibits the activity of immediate-early transcription unit 1

Ana C. Bratanich, Nancy D. Hanson, Clinton J. Jones

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory neurons of infected animals. Only one virus-encoded latency-related (LR) gene is expressed during a latent infection. The LR transcript overlaps immediate-early transcription unit 1 (lEtui) and is anti-sense with respect to IEtui. The transcriptional start site of the LR RNA was mapped to position 724 of the LR gene, downstream from two putative TATA elements. When LR promoter sequences were deleted from a plasmid containing IEtul and the LR gene, the resulting construct trans-activated the HSV-1 thymidine kinase (TK) promoter more efficiently than IEtui plus the LR gene. Cotransfection of a plasmid containing the intact LR gene with IEtu1 inhibited the ability of IEtu1 to trans-activate the TK promoter. These results imply that a LR gene product(s) repressed the trans-acting capacity of IEtu1.

Original languageEnglish (US)
Pages (from-to)988-991
Number of pages4
JournalVirology
Volume191
Issue number2
DOIs
StatePublished - Dec 1992

All Science Journal Classification (ASJC) codes

  • Virology

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