The Magnaporthe oryzae nitrooxidative stress response suppresses rice innate immunity during blast disease

Margarita Marroquin-Guzman, David Hartline, Janet D. Wright, Christian Elowsky, Travis J. Bourret, Richard A. Wilson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Understanding how microorganisms manipulate plant innate immunity and colonize host cells is a major goal of plant pathology. Here, we report that the fungal nitrooxidative stress response suppresses host defences to facilitate the growth and development of the important rice pathogen Magnaporthe oryzae in leaf cells. Nitronate monooxygenases encoded by NMO genes catalyse the oxidative denitrification of nitroalkanes. We show that the M. oryzae NMO2 gene is required for mitigating damaging lipid nitration under nitrooxidative stress conditions and, consequently, for using nitrate and nitrite as nitrogen sources. On plants, the Δnmo2 mutant strain penetrated host cuticles like wild type, but invasive hyphal growth in rice cells was restricted and elicited plant immune responses that included the formation of cellular deposits and a host reactive oxygen species burst. Development of the M. oryzae effector-secreting biotrophic interfacial complex (BIC) was misregulated in the Δnmo2 mutant. Inhibiting or quenching host reactive oxygen species suppressed rice innate immune responses and allowed the Δnmo2 mutant to grow and develop normally in infected cells. NMO2 is thus essential for mitigating nitrooxidative cellular damage and, in rice cells, maintaining redox balance to avoid triggering plant defences that impact M. oryzae growth and BIC development.

Original languageEnglish (US)
Article number17054
JournalNature Microbiology
Volume2
DOIs
StatePublished - Apr 18 2017

Fingerprint

Magnaporthe
Innate Immunity
Plant Immunity
Reactive Oxygen Species
Plant Pathology
Denitrification
Oryza
Growth
Nitrites
Mixed Function Oxygenases
Growth and Development
Nitrates
Genes
Oxidation-Reduction
Nitrogen
Lipids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology (medical)
  • Cell Biology
  • Genetics

Cite this

The Magnaporthe oryzae nitrooxidative stress response suppresses rice innate immunity during blast disease. / Marroquin-Guzman, Margarita; Hartline, David; Wright, Janet D.; Elowsky, Christian; Bourret, Travis J.; Wilson, Richard A.

In: Nature Microbiology, Vol. 2, 17054, 18.04.2017.

Research output: Contribution to journalArticle

Marroquin-Guzman, Margarita ; Hartline, David ; Wright, Janet D. ; Elowsky, Christian ; Bourret, Travis J. ; Wilson, Richard A. / The Magnaporthe oryzae nitrooxidative stress response suppresses rice innate immunity during blast disease. In: Nature Microbiology. 2017 ; Vol. 2.
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