The molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain

Geoffrey W. Coombs, Richard V. Goering, Kyra Y L Chua, Stefan Monecke, Benjamin P. Howden, Timothy P. Stinear, Ralf Ehricht, Frances G. O'Brien, Keryn J. Christiansen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In Australia the PVL - positive ST93-IV [2B], colloquially known as "Queensland CA-MRSA" has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage ΦSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable. The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVL-positive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known.

Original languageEnglish
Article numbere43037
JournalPLoS One
Volume7
Issue number8
DOIs
StatePublished - Aug 10 2012

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molecular epidemiology
Molecular Epidemiology
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Bacteriophages
Genes
Multilocus Sequence Typing
bacteriophages
virulence
Virulence
clones
Clone Cells
pulsed-field gel electrophoresis
Microarrays
Electrophoresis
DNA fingerprinting
Queensland
DNA Fingerprinting
pneumonia
genetic relationships

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Coombs, G. W., Goering, R. V., Chua, K. Y. L., Monecke, S., Howden, B. P., Stinear, T. P., ... Christiansen, K. J. (2012). The molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain. PLoS One, 7(8), [e43037]. https://doi.org/10.1371/journal.pone.0043037

The molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain. / Coombs, Geoffrey W.; Goering, Richard V.; Chua, Kyra Y L; Monecke, Stefan; Howden, Benjamin P.; Stinear, Timothy P.; Ehricht, Ralf; O'Brien, Frances G.; Christiansen, Keryn J.

In: PLoS One, Vol. 7, No. 8, e43037, 10.08.2012.

Research output: Contribution to journalArticle

Coombs, GW, Goering, RV, Chua, KYL, Monecke, S, Howden, BP, Stinear, TP, Ehricht, R, O'Brien, FG & Christiansen, KJ 2012, 'The molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain', PLoS One, vol. 7, no. 8, e43037. https://doi.org/10.1371/journal.pone.0043037
Coombs, Geoffrey W. ; Goering, Richard V. ; Chua, Kyra Y L ; Monecke, Stefan ; Howden, Benjamin P. ; Stinear, Timothy P. ; Ehricht, Ralf ; O'Brien, Frances G. ; Christiansen, Keryn J. / The molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain. In: PLoS One. 2012 ; Vol. 7, No. 8.
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