Abstract
The purpose of this study was to examine the morphological and functional development of the lateral wall of the scala media of the cochlea in miniature pigs; light and transmission electron microscopy and electrophysiology were used for this purpose. We showed that the lateral wall of the scala media of the cochlea appears at embryonic Day 21 (E21) when the cochlear duct begins to form. From E28 to E49, the lateral wall can be distinguished according to its position along the cochlea. At E56, cells in the lateral wall begin to differentiate into three different types. At E70, three cell types, marginal, intermediate and basal, can be clearly distinguished. At E91, the stria vascularis is adult-like and the organ of Corti is also morphologically mature. The average endocochlear potential measured from the second turn of the cochlea (at E98, postnatal Day 1 (P1), P13 and P30) was 71.4±2.5 (n≤7), 78.8±1.5 (n≤10), 77.3±2.3 (n≤10) and 78.0±2.1 mV (n≤10), respectively. Our results suggest that in miniature pigs the stria vascularis develops during the embryonic period, concurrent with maturation of the organ of Corti. The magnitude of the endocochlear potential reached its mature level when the stria vascularis was morphologically adult-like at E98. These findings provide a morphological and functional basis for future animal studies using the miniature pig model concerning the pathogenesis of various inner-ear diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 585-593 |
| Number of pages | 9 |
| Journal | Reproduction, Fertility and Development |
| Volume | 29 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2017 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Reproductive Medicine
- Molecular Biology
- Animal Science and Zoology
- Genetics
- Endocrinology
- Developmental Biology
Cite this
The morphological and functional development of the stria vascularis in miniature pigs. / Guo, Weiwei; Yi, Haijin; Yan, Zhang; Ren, Lili; Chen, Lei; Zhao, Li Dong; Ning, Yu; He, David Z.; Yang, Shi Ming.
In: Reproduction, Fertility and Development, Vol. 29, No. 3, 2017, p. 585-593.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The morphological and functional development of the stria vascularis in miniature pigs
AU - Guo, Weiwei
AU - Yi, Haijin
AU - Yan, Zhang
AU - Ren, Lili
AU - Chen, Lei
AU - Zhao, Li Dong
AU - Ning, Yu
AU - He, David Z.
AU - Yang, Shi Ming
PY - 2017
Y1 - 2017
N2 - The purpose of this study was to examine the morphological and functional development of the lateral wall of the scala media of the cochlea in miniature pigs; light and transmission electron microscopy and electrophysiology were used for this purpose. We showed that the lateral wall of the scala media of the cochlea appears at embryonic Day 21 (E21) when the cochlear duct begins to form. From E28 to E49, the lateral wall can be distinguished according to its position along the cochlea. At E56, cells in the lateral wall begin to differentiate into three different types. At E70, three cell types, marginal, intermediate and basal, can be clearly distinguished. At E91, the stria vascularis is adult-like and the organ of Corti is also morphologically mature. The average endocochlear potential measured from the second turn of the cochlea (at E98, postnatal Day 1 (P1), P13 and P30) was 71.4±2.5 (n≤7), 78.8±1.5 (n≤10), 77.3±2.3 (n≤10) and 78.0±2.1 mV (n≤10), respectively. Our results suggest that in miniature pigs the stria vascularis develops during the embryonic period, concurrent with maturation of the organ of Corti. The magnitude of the endocochlear potential reached its mature level when the stria vascularis was morphologically adult-like at E98. These findings provide a morphological and functional basis for future animal studies using the miniature pig model concerning the pathogenesis of various inner-ear diseases.
AB - The purpose of this study was to examine the morphological and functional development of the lateral wall of the scala media of the cochlea in miniature pigs; light and transmission electron microscopy and electrophysiology were used for this purpose. We showed that the lateral wall of the scala media of the cochlea appears at embryonic Day 21 (E21) when the cochlear duct begins to form. From E28 to E49, the lateral wall can be distinguished according to its position along the cochlea. At E56, cells in the lateral wall begin to differentiate into three different types. At E70, three cell types, marginal, intermediate and basal, can be clearly distinguished. At E91, the stria vascularis is adult-like and the organ of Corti is also morphologically mature. The average endocochlear potential measured from the second turn of the cochlea (at E98, postnatal Day 1 (P1), P13 and P30) was 71.4±2.5 (n≤7), 78.8±1.5 (n≤10), 77.3±2.3 (n≤10) and 78.0±2.1 mV (n≤10), respectively. Our results suggest that in miniature pigs the stria vascularis develops during the embryonic period, concurrent with maturation of the organ of Corti. The magnitude of the endocochlear potential reached its mature level when the stria vascularis was morphologically adult-like at E98. These findings provide a morphological and functional basis for future animal studies using the miniature pig model concerning the pathogenesis of various inner-ear diseases.
UR - http://www.scopus.com/inward/record.url?scp=85013393717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013393717&partnerID=8YFLogxK
U2 - 10.1071/RD15183
DO - 10.1071/RD15183
M3 - Article
C2 - 28442065
AN - SCOPUS:85013393717
VL - 29
SP - 585
EP - 593
JO - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
SN - 1031-3613
IS - 3
ER -