The nasal cavity is a route for prion infection in hamsters

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Abstract

Animals that naturally acquire the prion diseases have a well-developed olfactory sense that they utilize for a variety of basic behaviors. To assess the potential for the nasal cavity to serve as a point of entry for prion diseases, a small amount of prion-infected brain homogenate was placed inferior to the nostrils of hamsters, where it was immediately sniffed into the nasal cavity. Hamsters extranasally inoculated with the HY strain of transmissible mink encephalopathy (TME) agent had an incubation period that was not significantly different from per os inoculation of the same dose of the HY TME agent. However, the efficiency of the nasal route of inoculation was determined to be 10 to 100 times greater based on endpoint dilution analysis. Immunohistochemistry on tissues from hamsters killed at 2-week intervals after inoculation was used to identify the disease-associated form of the prion protein (PrPd) to determine the route of prion neuroinvasion. Nasal mucosa-associated lymphoid tissue and submandibular lymph nodes initially accumulated PrPd as early as 4 weeks postinfection. PrPd was first identified in cervical lymph nodes at 8 weeks, in the mesenteric lymph nodes, spleen, and Peyer's patches at 14 weeks, and in the tongue 20 weeks after inoculation. Surprisingly, there was no evidence of PrPd in olfactory epithelium or olfactory nerve fascicles at any time after inoculation. Therefore, the HY TME agent did not enter the central nervous system via the olfactory nerve; instead, PrPd accumulated in elements of the cranial lymphoreticular system prior to neuroinvasion.

Original languageEnglish
Pages (from-to)4482-4491
Number of pages10
JournalJournal of Virology
Volume81
Issue number9
DOIs
StatePublished - May 2007

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transmissible mink encephalopathy
Mink
nasal cavity
Prions
Nasal Cavity
prions
Brain Diseases
hamsters
Cricetinae
Olfactory Nerve
lymph nodes
Prion Diseases
prion diseases
Lymph Nodes
nerve tissue
Infection
infection
nasal mucosa
Olfactory Mucosa
Peyer's Patches

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

The nasal cavity is a route for prion infection in hamsters. / Kincaid, Anthony; Bartz, Jason C.

In: Journal of Virology, Vol. 81, No. 9, 05.2007, p. 4482-4491.

Research output: Contribution to journalArticle

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