The OpdQ porin of Pseudomonas aeruginosa is regulated by environmental signals associated with cystic fibrosis including nitrate-induced regulation involving the NarXL two-component system

Randal C. Fowler, Nancy D. Hanson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is a versatile opportunistic pathogen that causes chronic infections in immunocompromised hosts. Multiple porins modulate outer membrane permeability under various environmental conditions. The lung environment of cystic fibrosis (CF) patients is unique with changes occurring in nutrient availability, osmolarity, and oxygen content. Although P. aeruginosa gene expression is modified under these conditions, little is known about how they influence porin regulation. In this study, we evaluated the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, with regard to oxygen, nitrate, and/or NaCl levels. We demonstrated using promoter::fusion clones of P. aeruginosa PAO1 and clinical strains collected from CF patients that OpdQ was transcriptionally repressed under low oxygen but increased in the presence of nitrate. The nitrate-induced regulation of OpdQ was found to be dependent on the transcription factor NarL via the NarXL two-component system. In addition, NaCl-induced osmotic stress increased OpdQ production among most of the clinical strains evaluated. In conclusion, these data identify for the first time that specific environmental cues associated with the CF microenvironment influence porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system of P. aeruginosa. Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of causing a variety of severe and chronic infections in immunocompromised hosts. In this study, we show that the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, is dependent on oxygen, nitrate, and/or NaCl levels. Our results indicate for the first time that specific environmental signals effect porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system in P. aeruginosa.

Original languageEnglish
Pages (from-to)967-982
Number of pages16
JournalMicrobiologyOpen
Volume4
Issue number6
DOIs
StatePublished - Dec 1 2015

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Porins
Cystic Fibrosis
Nitrates
Pseudomonas aeruginosa
Oxygen
Immunocompromised Host
Membranes
Osmotic Pressure
Infection
Osmolar Concentration
Cues
Permeability
Transcription Factors
Clone Cells
Gene Expression
Food
Lung

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

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title = "The OpdQ porin of Pseudomonas aeruginosa is regulated by environmental signals associated with cystic fibrosis including nitrate-induced regulation involving the NarXL two-component system",
abstract = "Pseudomonas aeruginosa is a versatile opportunistic pathogen that causes chronic infections in immunocompromised hosts. Multiple porins modulate outer membrane permeability under various environmental conditions. The lung environment of cystic fibrosis (CF) patients is unique with changes occurring in nutrient availability, osmolarity, and oxygen content. Although P. aeruginosa gene expression is modified under these conditions, little is known about how they influence porin regulation. In this study, we evaluated the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, with regard to oxygen, nitrate, and/or NaCl levels. We demonstrated using promoter::fusion clones of P. aeruginosa PAO1 and clinical strains collected from CF patients that OpdQ was transcriptionally repressed under low oxygen but increased in the presence of nitrate. The nitrate-induced regulation of OpdQ was found to be dependent on the transcription factor NarL via the NarXL two-component system. In addition, NaCl-induced osmotic stress increased OpdQ production among most of the clinical strains evaluated. In conclusion, these data identify for the first time that specific environmental cues associated with the CF microenvironment influence porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system of P. aeruginosa. Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of causing a variety of severe and chronic infections in immunocompromised hosts. In this study, we show that the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, is dependent on oxygen, nitrate, and/or NaCl levels. Our results indicate for the first time that specific environmental signals effect porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system in P. aeruginosa.",
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AU - Fowler, Randal C.

AU - Hanson, Nancy D.

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N2 - Pseudomonas aeruginosa is a versatile opportunistic pathogen that causes chronic infections in immunocompromised hosts. Multiple porins modulate outer membrane permeability under various environmental conditions. The lung environment of cystic fibrosis (CF) patients is unique with changes occurring in nutrient availability, osmolarity, and oxygen content. Although P. aeruginosa gene expression is modified under these conditions, little is known about how they influence porin regulation. In this study, we evaluated the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, with regard to oxygen, nitrate, and/or NaCl levels. We demonstrated using promoter::fusion clones of P. aeruginosa PAO1 and clinical strains collected from CF patients that OpdQ was transcriptionally repressed under low oxygen but increased in the presence of nitrate. The nitrate-induced regulation of OpdQ was found to be dependent on the transcription factor NarL via the NarXL two-component system. In addition, NaCl-induced osmotic stress increased OpdQ production among most of the clinical strains evaluated. In conclusion, these data identify for the first time that specific environmental cues associated with the CF microenvironment influence porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system of P. aeruginosa. Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of causing a variety of severe and chronic infections in immunocompromised hosts. In this study, we show that the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, is dependent on oxygen, nitrate, and/or NaCl levels. Our results indicate for the first time that specific environmental signals effect porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system in P. aeruginosa.

AB - Pseudomonas aeruginosa is a versatile opportunistic pathogen that causes chronic infections in immunocompromised hosts. Multiple porins modulate outer membrane permeability under various environmental conditions. The lung environment of cystic fibrosis (CF) patients is unique with changes occurring in nutrient availability, osmolarity, and oxygen content. Although P. aeruginosa gene expression is modified under these conditions, little is known about how they influence porin regulation. In this study, we evaluated the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, with regard to oxygen, nitrate, and/or NaCl levels. We demonstrated using promoter::fusion clones of P. aeruginosa PAO1 and clinical strains collected from CF patients that OpdQ was transcriptionally repressed under low oxygen but increased in the presence of nitrate. The nitrate-induced regulation of OpdQ was found to be dependent on the transcription factor NarL via the NarXL two-component system. In addition, NaCl-induced osmotic stress increased OpdQ production among most of the clinical strains evaluated. In conclusion, these data identify for the first time that specific environmental cues associated with the CF microenvironment influence porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system of P. aeruginosa. Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of causing a variety of severe and chronic infections in immunocompromised hosts. In this study, we show that the regulation of the outer membrane porin OpdQ, a member of the OprD family of porins, is dependent on oxygen, nitrate, and/or NaCl levels. Our results indicate for the first time that specific environmental signals effect porin regulation, and that the nitrate-induced regulation of OpdQ is associated with nitrate metabolism via the NarXL two-component system in P. aeruginosa.

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