The profile of bile acids and their sulfate metabolites in human urine and serum

Sai Praneeth R Bathena, Sandeep Mukherjee, Marco Olivera, Yazen Alnouti

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The role of sulfation in ameliorating the hepatotoxicity of bile acids (BAs) in humans remains unknown due to the lack of proper analytical methods to quantify individual BAs and their sulfate metabolites in biological tissues and fluids. To this end, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to characterize the detailed BA profile in human urine and serum. The limit of quantification was 1. ng/mL and baseline separation of all analytes was achieved within in a run time of 32. min. The method was validated over the dynamic range of 1-1000. ng/mL. The LC-MS/MS method was more accurate, precise, and selective than the commercially available kits for the quantification of sulfated and unsulfated BAs, and the indirect quantification of individual sulfated BAs after solvolysis. The LC-MS/MS method was applied to characterize the BA profile in urine and serum of healthy subjects. Thirty three percent of serum BAs were sulfated, whereas 89% of urinary BAs existed in the sulfate form, indicating the role of sulfation in enhancing the urinary excretion of BAs. The percentage of sulfation of individual BAs increased with the decrease in the number of hydroxyl groups indicating the role of sulfation in the detoxification of the more hydrophobic and toxic BA species. Eighty percent of urinary BAs and 55% of serum BAs were present in the glycine-amidated form, whereas 8% of urinary BAs and 13% of serum BAs existed in the taurine-amidated form.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume942-943
DOIs
StatePublished - Dec 30 2013
Externally publishedYes

Fingerprint

Metabolites
Bile Acids and Salts
Urine
Serum
bile acid sulfates
Detoxification
Poisons
Taurine
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Hydroxyl Radical
Glycine
Sulfates
Mass spectrometry
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

The profile of bile acids and their sulfate metabolites in human urine and serum. / Bathena, Sai Praneeth R; Mukherjee, Sandeep; Olivera, Marco; Alnouti, Yazen.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 942-943, 30.12.2013, p. 53-62.

Research output: Contribution to journalArticle

@article{b6b04963dd2647869253d51a78915128,
title = "The profile of bile acids and their sulfate metabolites in human urine and serum",
abstract = "The role of sulfation in ameliorating the hepatotoxicity of bile acids (BAs) in humans remains unknown due to the lack of proper analytical methods to quantify individual BAs and their sulfate metabolites in biological tissues and fluids. To this end, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to characterize the detailed BA profile in human urine and serum. The limit of quantification was 1. ng/mL and baseline separation of all analytes was achieved within in a run time of 32. min. The method was validated over the dynamic range of 1-1000. ng/mL. The LC-MS/MS method was more accurate, precise, and selective than the commercially available kits for the quantification of sulfated and unsulfated BAs, and the indirect quantification of individual sulfated BAs after solvolysis. The LC-MS/MS method was applied to characterize the BA profile in urine and serum of healthy subjects. Thirty three percent of serum BAs were sulfated, whereas 89{\%} of urinary BAs existed in the sulfate form, indicating the role of sulfation in enhancing the urinary excretion of BAs. The percentage of sulfation of individual BAs increased with the decrease in the number of hydroxyl groups indicating the role of sulfation in the detoxification of the more hydrophobic and toxic BA species. Eighty percent of urinary BAs and 55{\%} of serum BAs were present in the glycine-amidated form, whereas 8{\%} of urinary BAs and 13{\%} of serum BAs existed in the taurine-amidated form.",
author = "Bathena, {Sai Praneeth R} and Sandeep Mukherjee and Marco Olivera and Yazen Alnouti",
year = "2013",
month = "12",
day = "30",
doi = "10.1016/j.jchromb.2013.10.019",
language = "English",
volume = "942-943",
pages = "53--62",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",

}

TY - JOUR

T1 - The profile of bile acids and their sulfate metabolites in human urine and serum

AU - Bathena, Sai Praneeth R

AU - Mukherjee, Sandeep

AU - Olivera, Marco

AU - Alnouti, Yazen

PY - 2013/12/30

Y1 - 2013/12/30

N2 - The role of sulfation in ameliorating the hepatotoxicity of bile acids (BAs) in humans remains unknown due to the lack of proper analytical methods to quantify individual BAs and their sulfate metabolites in biological tissues and fluids. To this end, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to characterize the detailed BA profile in human urine and serum. The limit of quantification was 1. ng/mL and baseline separation of all analytes was achieved within in a run time of 32. min. The method was validated over the dynamic range of 1-1000. ng/mL. The LC-MS/MS method was more accurate, precise, and selective than the commercially available kits for the quantification of sulfated and unsulfated BAs, and the indirect quantification of individual sulfated BAs after solvolysis. The LC-MS/MS method was applied to characterize the BA profile in urine and serum of healthy subjects. Thirty three percent of serum BAs were sulfated, whereas 89% of urinary BAs existed in the sulfate form, indicating the role of sulfation in enhancing the urinary excretion of BAs. The percentage of sulfation of individual BAs increased with the decrease in the number of hydroxyl groups indicating the role of sulfation in the detoxification of the more hydrophobic and toxic BA species. Eighty percent of urinary BAs and 55% of serum BAs were present in the glycine-amidated form, whereas 8% of urinary BAs and 13% of serum BAs existed in the taurine-amidated form.

AB - The role of sulfation in ameliorating the hepatotoxicity of bile acids (BAs) in humans remains unknown due to the lack of proper analytical methods to quantify individual BAs and their sulfate metabolites in biological tissues and fluids. To this end, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to characterize the detailed BA profile in human urine and serum. The limit of quantification was 1. ng/mL and baseline separation of all analytes was achieved within in a run time of 32. min. The method was validated over the dynamic range of 1-1000. ng/mL. The LC-MS/MS method was more accurate, precise, and selective than the commercially available kits for the quantification of sulfated and unsulfated BAs, and the indirect quantification of individual sulfated BAs after solvolysis. The LC-MS/MS method was applied to characterize the BA profile in urine and serum of healthy subjects. Thirty three percent of serum BAs were sulfated, whereas 89% of urinary BAs existed in the sulfate form, indicating the role of sulfation in enhancing the urinary excretion of BAs. The percentage of sulfation of individual BAs increased with the decrease in the number of hydroxyl groups indicating the role of sulfation in the detoxification of the more hydrophobic and toxic BA species. Eighty percent of urinary BAs and 55% of serum BAs were present in the glycine-amidated form, whereas 8% of urinary BAs and 13% of serum BAs existed in the taurine-amidated form.

UR - http://www.scopus.com/inward/record.url?scp=84887399153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887399153&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2013.10.019

DO - 10.1016/j.jchromb.2013.10.019

M3 - Article

VL - 942-943

SP - 53

EP - 62

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -