Abstract
1. 1. The quantitative analysis of biliary lipids has been well characterized, however, there is little data related to the qualitative and quantitative analysis of phospholipids and conjugated bile salts for the prairie dog. 2. 2. Gallbladder and hepatic bile were collected and the bile salts in each sample were analyzed by first fractionating them into unconjugated, glycoconjugated, and tauroconjugated bile salts. These fractions were further resolved by using HPLC techniques. 3. 3. The data has shown that cholate exists predominately as the tauroconjugate and is the major bile salt, whereas chenodeoxycholate is rarely conjugated. 4. 4. The qualitative analysis of biliary phospholipids by thin layer chromatography has shown a remarkable similarity to that of humans. Phosphatidylcholine was found to be the major phospholipid followed by phosphatidylethanolamine with trace amounts of lysophosphatidylcholine and phosphatidylserine.
| Original language | English |
|---|---|
| Pages (from-to) | 521-526 |
| Number of pages | 6 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 97 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1990 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physiology
Cite this
The quantitative and qualitative analysis for biliary lipids in the prairie dog Cynomys ludovicianus. / Broughton, George; Tseng, Anthony; Fitzgibbons, Robert Joseph; Fishkin, Arthur F.; Rongone, Edward L.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 97, No. 3, 1990, p. 521-526.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The quantitative and qualitative analysis for biliary lipids in the prairie dog Cynomys ludovicianus
AU - Broughton, George
AU - Tseng, Anthony
AU - Fitzgibbons, Robert Joseph
AU - Fishkin, Arthur F.
AU - Rongone, Edward L.
PY - 1990
Y1 - 1990
N2 - 1. 1. The quantitative analysis of biliary lipids has been well characterized, however, there is little data related to the qualitative and quantitative analysis of phospholipids and conjugated bile salts for the prairie dog. 2. 2. Gallbladder and hepatic bile were collected and the bile salts in each sample were analyzed by first fractionating them into unconjugated, glycoconjugated, and tauroconjugated bile salts. These fractions were further resolved by using HPLC techniques. 3. 3. The data has shown that cholate exists predominately as the tauroconjugate and is the major bile salt, whereas chenodeoxycholate is rarely conjugated. 4. 4. The qualitative analysis of biliary phospholipids by thin layer chromatography has shown a remarkable similarity to that of humans. Phosphatidylcholine was found to be the major phospholipid followed by phosphatidylethanolamine with trace amounts of lysophosphatidylcholine and phosphatidylserine.
AB - 1. 1. The quantitative analysis of biliary lipids has been well characterized, however, there is little data related to the qualitative and quantitative analysis of phospholipids and conjugated bile salts for the prairie dog. 2. 2. Gallbladder and hepatic bile were collected and the bile salts in each sample were analyzed by first fractionating them into unconjugated, glycoconjugated, and tauroconjugated bile salts. These fractions were further resolved by using HPLC techniques. 3. 3. The data has shown that cholate exists predominately as the tauroconjugate and is the major bile salt, whereas chenodeoxycholate is rarely conjugated. 4. 4. The qualitative analysis of biliary phospholipids by thin layer chromatography has shown a remarkable similarity to that of humans. Phosphatidylcholine was found to be the major phospholipid followed by phosphatidylethanolamine with trace amounts of lysophosphatidylcholine and phosphatidylserine.
UR - http://www.scopus.com/inward/record.url?scp=0025259978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025259978&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(90)90153-K
DO - 10.1016/0305-0491(90)90153-K
M3 - Article
VL - 97
SP - 521
EP - 526
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 3
ER -