The role of ganglioside GM3 in the modulation of conformation and activity of sarcoplasmic reticulum CA2+-ATPase

F. Y. Yang, L. H. Wang, X. Y. Yang, Z. C. Tsui, Yaping Tu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rabbit sarcoplasmic reticulum does contain trace amounts of gangliosides, and the main species is GM3. Incorporation of GM3 into the SR vesicles or addition of it to the soybean phospholipid used for reconstitution of proteoliposomes obviously increased ATP hydrolysis, as well as, Ca2+ uptake activity of sarcoplasmic reticulum Ca2+-ATPase. Conformation changes of Ca2+-ATPase induced by GM3 were also observed by circular dichroism, intrinsic fluorescence and fluorescence quenching measurements.

Original languageEnglish
Pages (from-to)137-146
Number of pages10
JournalBiophysical Chemistry
Volume68
Issue number1-3
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

sarcoplasmic reticulum
G(M3) Ganglioside
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Adenosine Triphosphatases
Conformations
Fluorescence
Modulation
modulation
fluorescence
soybeans
adenosine triphosphate
Gangliosides
rabbits
Circular Dichroism
Soybeans
dichroism
hydrolysis
Quenching
Hydrolysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Physical and Theoretical Chemistry

Cite this

The role of ganglioside GM3 in the modulation of conformation and activity of sarcoplasmic reticulum CA2+-ATPase. / Yang, F. Y.; Wang, L. H.; Yang, X. Y.; Tsui, Z. C.; Tu, Yaping.

In: Biophysical Chemistry, Vol. 68, No. 1-3, 10.1997, p. 137-146.

Research output: Contribution to journalArticle

Yang, F. Y. ; Wang, L. H. ; Yang, X. Y. ; Tsui, Z. C. ; Tu, Yaping. / The role of ganglioside GM3 in the modulation of conformation and activity of sarcoplasmic reticulum CA2+-ATPase. In: Biophysical Chemistry. 1997 ; Vol. 68, No. 1-3. pp. 137-146.
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