The role of ganglioside GM3 in the modulation of conformation and activity of sarcoplasmic reticulum CA2+-ATPase

F. Y. Yang; L. H. Wang; X. Y. Yang; Z. C. Tsui; Yaping Tu

doi:10.1016/S0301-4622(97)00025-2

The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase

F. Y. Yang, L. H. Wang, X. Y. Yang, Z. C. Tsui, Yaping Tu

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Rabbit sarcoplasmic reticulum does contain trace amounts of gangliosides, and the main species is GM₃. Incorporation of GM₃ into the SR vesicles or addition of it to the soybean phospholipid used for reconstitution of proteoliposomes obviously increased ATP hydrolysis, as well as, Ca²⁺ uptake activity of sarcoplasmic reticulum Ca²⁺-ATPase. Conformation changes of Ca²⁺-ATPase induced by GM₃ were also observed by circular dichroism, intrinsic fluorescence and fluorescence quenching measurements.

Original language	English
Pages (from-to)	137-146
Number of pages	10
Journal	Biophysical Chemistry
Volume	68
Issue number	1-3
DOIs	https://doi.org/10.1016/S0301-4622(97)00025-2
State	Published - Oct 1997
Externally published	Yes

Fingerprint

sarcoplasmic reticulum

G(M3) Ganglioside

Calcium-Transporting ATPases

Sarcoplasmic Reticulum

Adenosine Triphosphatases

Conformations

Fluorescence

Modulation

modulation

fluorescence

soybeans

adenosine triphosphate

Gangliosides

rabbits

Circular Dichroism

Soybeans

dichroism

hydrolysis

Quenching

Hydrolysis

All Science Journal Classification (ASJC) codes

Biochemistry
Biophysics
Physical and Theoretical Chemistry

Cite this

Yang, F. Y., Wang, L. H., Yang, X. Y., Tsui, Z. C., & Tu, Y. (1997). The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase. Biophysical Chemistry, 68(1-3), 137-146. https://doi.org/10.1016/S0301-4622(97)00025-2

The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase. / Yang, F. Y.; Wang, L. H.; Yang, X. Y.; Tsui, Z. C.; Tu, Yaping.

In: Biophysical Chemistry, Vol. 68, No. 1-3, 10.1997, p. 137-146.

Research output: Contribution to journal › Article

Yang, FY, Wang, LH, Yang, XY, Tsui, ZC & Tu, Y 1997, 'The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase', Biophysical Chemistry, vol. 68, no. 1-3, pp. 137-146. https://doi.org/10.1016/S0301-4622(97)00025-2

Yang FY, Wang LH, Yang XY, Tsui ZC, Tu Y. The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase. Biophysical Chemistry. 1997 Oct;68(1-3):137-146. https://doi.org/10.1016/S0301-4622(97)00025-2

Yang, F. Y. ; Wang, L. H. ; Yang, X. Y. ; Tsui, Z. C. ; Tu, Yaping. / The role of ganglioside GM₃ in the modulation of conformation and activity of sarcoplasmic reticulum CA²⁺-ATPase. In: Biophysical Chemistry. 1997 ; Vol. 68, No. 1-3. pp. 137-146.

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10.1016/S0301-4622(97)00025-2