Thermosensitive gel containing cellulose acetate phthalate-efavirenz combination nanoparticles for prevention of HIV-1 infection

Abhijit A. Date, Annemarie Shibata, Emily McMullen, Krista La Bruzzo, Patrick Bruck, Michael A. Belshan, You Zhou, Christopher J. Destache

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The objective of this investigation was to develop and evaluate a nano-microbicide containing a combination of cellulose acetate phthalate (HIV-1 entry inhibitor) and efavirenz (anti-HIV agent) for HIV prophylaxis. Cellulose acetate phthalateefavirenz combination nanoparticles (CAP-EFV-NPs) were fabricated by the nanoprecipitation method and were characterized for particle size, zeta potential and encapsulation efficiency of efavirenz. CAP-EFV-NPs were incorporated into a thermosensitive gel (CAP-EFV-NP-Gel). CAP-EFV-NPs, CAP-EFV-NP-Gel and efavirenz solution were evaluated for cytotoxicity to HeLa cells and for in vitro short-term (1-day) and long-term (3-day) prophylaxis against HIV-1 infection in TZM-bl cells. CAP-EFV-NPs had size 98%. CAP-EFV-NPs and CAP-EFV-NP-Gel were significantly less toxic (P <0.01) to HeLa cells as compared to efavirenz solution. CAP-EFV-NPs showed significantly higher prophylactic activity (P

Original languageEnglish
Pages (from-to)416-427
Number of pages12
JournalJournal of Biomedical Nanotechnology
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science
  • Medicine(all)

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