Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis

Andrzej Rajca, Qingsheng Li, Abhijit Date, Michael A. Belshan, Christopher J. Destache

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Nucleoside reverse transcriptase inhibitors (NRTIs) have shown efficacy as pre-exposure prophylaxis (PrEP). The research goal is conjugating NRTIs to biodegradable polymer poly (lactic-co-glycolic acid; PLGA) by an amide bond. NRTIs-PLGA conjugates were chemically activated and conjugated to free amine group of emtricitabine (FTC) and lamivudine (3TC). The conjugates were made into nanoparticles (NPs) and incorporated into a thermosensitive gel for vaginal PrEP. PLGA-FTC and PLGA-3TC NPs were <200nm and zeta potential of-13.7 and -14.1 mV, respectively. The conjugated NPs were not toxic to HeLa cells up to 100 mcg/ml. Thermosensitive gel containing fluorescent NPs fabricated similarly and administered vaginally, were seen in submucosa for up to 5 days. FTC-NPs showed similar EC50 compared to FTC solution. This thermosensitive gel would be a novel vaginal sustained-release PrEP option.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages293-296
Number of pages4
Volume3
StatePublished - 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Other

OtherNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Fingerprint

Foams and Jellies Vaginal Creams
Reverse Transcriptase Inhibitors
Nucleosides
Nanoparticles
HIV
Lamivudine
Gels
Poisons
HeLa Cells
Amides
Amines
Polymers
polylactic acid-polyglycolic acid copolymer
Research
Pre-Exposure Prophylaxis

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Rajca, A., Li, Q., Date, A., Belshan, M. A., & Destache, C. J. (2013). Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (Vol. 3, pp. 293-296)

Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis. / Rajca, Andrzej; Li, Qingsheng; Date, Abhijit; Belshan, Michael A.; Destache, Christopher J.

Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. p. 293-296.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rajca, A, Li, Q, Date, A, Belshan, MA & Destache, CJ 2013, Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis. in Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. vol. 3, pp. 293-296, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, Washington, DC, United States, 5/12/13.
Rajca A, Li Q, Date A, Belshan MA, Destache CJ. Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3. 2013. p. 293-296
Rajca, Andrzej ; Li, Qingsheng ; Date, Abhijit ; Belshan, Michael A. ; Destache, Christopher J. / Thermosensitive vaginal gel containing PLGA-NRTI conjugated nanoparticles for HIV prophylaxis. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. pp. 293-296
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