Abstract
Matrix metalloproteinases (MMPs) are important mediators of neural crest (NC) cell migration. Here, we examine the distribution of tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3 and test whether manipulating TIMP levels alters chicken cardiac NC cell migration. TIMP-2 mRNA is expressed at stage 11 in the neural epithelium and only in migrating cardiac NC cells. TIMP-3 mRNA is expressed only in the notochord at stage 8 and later in the outflow tract myocardium. Exogenous TIMP-2 increases NC motility in vitro at low concentrations but has no effect when concentrations are increased. In vitro, NC cells express membrane type-1 matrix metalloproteinase (MT1-MMP) and TIMP-2 and they secrete and activate proMMP-2. Antisense TIMP-2 oligonucleotides block proMMP-2 activation, decrease NC cell migration from explants, and perturb NC morphogenesis in ovo. Because TIMP-2 is required for activation of proPMP-2 by MT1-MMP, this finding suggests TIMP-2 expression by cardiac NC cells initiates proMMP-2 activation important for their migration.
| Original language | English |
|---|---|
| Pages (from-to) | 709-719 |
| Number of pages | 11 |
| Journal | Developmental Dynamics |
| Volume | 231 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2004 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Cell Biology
- Developmental Biology
Cite this
Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) expression during cardiac neural crest cell migration and its role in ProMMP-2 activation. / Cantemir, V.; Cai, D. H.; Reedy, Mark; Brauer, P. R.
In: Developmental Dynamics, Vol. 231, No. 4, 12.2004, p. 709-719.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) expression during cardiac neural crest cell migration and its role in ProMMP-2 activation
AU - Cantemir, V.
AU - Cai, D. H.
AU - Reedy, Mark
AU - Brauer, P. R.
PY - 2004/12
Y1 - 2004/12
N2 - Matrix metalloproteinases (MMPs) are important mediators of neural crest (NC) cell migration. Here, we examine the distribution of tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3 and test whether manipulating TIMP levels alters chicken cardiac NC cell migration. TIMP-2 mRNA is expressed at stage 11 in the neural epithelium and only in migrating cardiac NC cells. TIMP-3 mRNA is expressed only in the notochord at stage 8 and later in the outflow tract myocardium. Exogenous TIMP-2 increases NC motility in vitro at low concentrations but has no effect when concentrations are increased. In vitro, NC cells express membrane type-1 matrix metalloproteinase (MT1-MMP) and TIMP-2 and they secrete and activate proMMP-2. Antisense TIMP-2 oligonucleotides block proMMP-2 activation, decrease NC cell migration from explants, and perturb NC morphogenesis in ovo. Because TIMP-2 is required for activation of proPMP-2 by MT1-MMP, this finding suggests TIMP-2 expression by cardiac NC cells initiates proMMP-2 activation important for their migration.
AB - Matrix metalloproteinases (MMPs) are important mediators of neural crest (NC) cell migration. Here, we examine the distribution of tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3 and test whether manipulating TIMP levels alters chicken cardiac NC cell migration. TIMP-2 mRNA is expressed at stage 11 in the neural epithelium and only in migrating cardiac NC cells. TIMP-3 mRNA is expressed only in the notochord at stage 8 and later in the outflow tract myocardium. Exogenous TIMP-2 increases NC motility in vitro at low concentrations but has no effect when concentrations are increased. In vitro, NC cells express membrane type-1 matrix metalloproteinase (MT1-MMP) and TIMP-2 and they secrete and activate proMMP-2. Antisense TIMP-2 oligonucleotides block proMMP-2 activation, decrease NC cell migration from explants, and perturb NC morphogenesis in ovo. Because TIMP-2 is required for activation of proPMP-2 by MT1-MMP, this finding suggests TIMP-2 expression by cardiac NC cells initiates proMMP-2 activation important for their migration.
UR - http://www.scopus.com/inward/record.url?scp=11144268173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11144268173&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20171
DO - 10.1002/dvdy.20171
M3 - Article
C2 - 15497141
AN - SCOPUS:11144268173
VL - 231
SP - 709
EP - 719
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 4
ER -