Total error profiling of a proinsulin time-resolved fluorescence immunoassay

P. E. De Pauw; R. B. Mackin; P. Goubert; C. Van Schravendijk; F. K. Gorus

doi:10.1016/j.jchromb.2008.11.024

Total error profiling of a proinsulin time-resolved fluorescence immunoassay

P. E. De Pauw, R. B. Mackin, P. Goubert, C. Van Schravendijk, F. K. Gorus

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).

Original language	English (US)
Pages (from-to)	2403-2406
Number of pages	4
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	23
DOIs	https://doi.org/10.1016/j.jchromb.2008.11.024
State	Published - Sep 1 2009

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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title = "Total error profiling of a proinsulin time-resolved fluorescence immunoassay",

abstract = "We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).",

author = "{De Pauw}, {P. E.} and Mackin, {R. B.} and P. Goubert and {Van Schravendijk}, C. and Gorus, {F. K.}",

note = "Funding Information: The present work was supported by grants from the Juvenile Diabetes Research Foundation (JDRF, Grant 4-2005-1327), The Belgian Research Council (FWO Grants G.0517.04 and G. 0311.07), the Research Council from the Brussels Free University (OZR 1150 and 1449) and the Scientific Fund Willy Gepts (UZ Brussel). ",

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AU - De Pauw, P. E.

AU - Mackin, R. B.

AU - Goubert, P.

AU - Van Schravendijk, C.

AU - Gorus, F. K.

N1 - Funding Information: The present work was supported by grants from the Juvenile Diabetes Research Foundation (JDRF, Grant 4-2005-1327), The Belgian Research Council (FWO Grants G.0517.04 and G. 0311.07), the Research Council from the Brussels Free University (OZR 1150 and 1449) and the Scientific Fund Willy Gepts (UZ Brussel).

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AB - We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).

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Total error profiling of a proinsulin time-resolved fluorescence immunoassay

Abstract

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