Total error profiling of a proinsulin time-resolved fluorescence immunoassay

P. E. De Pauw, Robert Mackin, P. Goubert, C. Van Schravendijk, F. K. Gorus

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).

Original languageEnglish
Pages (from-to)2403-2406
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number23
DOIs
StatePublished - Sep 1 2009

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Proinsulin
Immunoassay
Assays
Immunosorbents
Fluorescence
Enzyme-Linked Immunosorbent Assay
C-Peptide
Focus Groups
Enzymes
Ligands

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Total error profiling of a proinsulin time-resolved fluorescence immunoassay. / De Pauw, P. E.; Mackin, Robert; Goubert, P.; Van Schravendijk, C.; Gorus, F. K.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 23, 01.09.2009, p. 2403-2406.

Research output: Contribution to journalArticle

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