Total error profiling of a proinsulin time-resolved fluorescence immunoassay

P. E. De Pauw; R. B. Mackin; P. Goubert; C. Van Schravendijk; F. K. Gorus

doi:10.1016/j.jchromb.2008.11.024

Total error profiling of a proinsulin time-resolved fluorescence immunoassay

P. E. De Pauw, R. B. Mackin, P. Goubert, C. Van Schravendijk, F. K. Gorus

Department of Biomedical Sciences

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).

Original language	English (US)
Pages (from-to)	2403-2406
Number of pages	4
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	23
DOIs	https://doi.org/10.1016/j.jchromb.2008.11.024
State	Published - Sep 1 2009

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All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

Cite this

De Pauw, P. E., Mackin, R. B., Goubert, P., Van Schravendijk, C., & Gorus, F. K. (2009). Total error profiling of a proinsulin time-resolved fluorescence immunoassay. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(23), 2403-2406. https://doi.org/10.1016/j.jchromb.2008.11.024

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10.1016/j.jchromb.2008.11.024