Abstract
We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).
| Original language | English |
|---|---|
| Pages (from-to) | 2403-2406 |
| Number of pages | 4 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 877 |
| Issue number | 23 |
| DOIs | |
| State | Published - Sep 1 2009 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Analytical Chemistry
- Cell Biology
- Clinical Biochemistry
Cite this
Total error profiling of a proinsulin time-resolved fluorescence immunoassay. / De Pauw, P. E.; Mackin, Robert; Goubert, P.; Van Schravendijk, C.; Gorus, F. K.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 23, 01.09.2009, p. 2403-2406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Total error profiling of a proinsulin time-resolved fluorescence immunoassay
AU - De Pauw, P. E.
AU - Mackin, Robert
AU - Goubert, P.
AU - Van Schravendijk, C.
AU - Gorus, F. K.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).
AB - We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L).
UR - http://www.scopus.com/inward/record.url?scp=67650170835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650170835&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2008.11.024
DO - 10.1016/j.jchromb.2008.11.024
M3 - Article
VL - 877
SP - 2403
EP - 2406
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 23
ER -