TY - GEN
T1 - Trafficking of aminoglycosides into endolymph in vivo
AU - Wang, Qi
AU - Steyger, Peter S.
N1 - Funding Information:
Funded by National Institute of Deafness and other Communication Disorders (grants DC004555 and DC005983).
Publisher Copyright:
© 2008 World Scientific Publishing Co. All rights reserved.
PY - 2008
Y1 - 2008
N2 - In vitro, aminoglycosides increase the stiffness of cochlear hair cell stereocilia, altering bundle motion and transduction kinetics. Aminoglycosides also permeate the mechanosensitive transduction channel and rapidly initiate cytotoxicity in hair cells. If these effects occur in vivo, aminoglycosides would need to enter endolymph. The most direct route for systemically-administered aminoglycosides to enter endolymph is by trafficking from strial capillaries across the stria vascularis. An as-yet-unidentified active transporter is required to translocate aminoglycosides from the intra-strial space into the cytoplasm of marginal cells. Once in marginal cells, aminoglycosides would passively flow down the electrochemical gradient into endolymph. We present data that support a trans-strial trafficking route of aminoglycosides into endolymph, where they can then interfere with the mechanosensitive hair bundles.
AB - In vitro, aminoglycosides increase the stiffness of cochlear hair cell stereocilia, altering bundle motion and transduction kinetics. Aminoglycosides also permeate the mechanosensitive transduction channel and rapidly initiate cytotoxicity in hair cells. If these effects occur in vivo, aminoglycosides would need to enter endolymph. The most direct route for systemically-administered aminoglycosides to enter endolymph is by trafficking from strial capillaries across the stria vascularis. An as-yet-unidentified active transporter is required to translocate aminoglycosides from the intra-strial space into the cytoplasm of marginal cells. Once in marginal cells, aminoglycosides would passively flow down the electrochemical gradient into endolymph. We present data that support a trans-strial trafficking route of aminoglycosides into endolymph, where they can then interfere with the mechanosensitive hair bundles.
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U2 - 10.1142/9789812833785_0072
DO - 10.1142/9789812833785_0072
M3 - Conference contribution
AN - SCOPUS:85066977581
T3 - Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008
SP - 439
EP - 443
BT - Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008
A2 - Cooper, Nigel P.
A2 - Kemp, David T.
PB - World Scientific Publishing Co. Pte Ltd
T2 - 10th International Workshop on the Mechanics of Hearing - Concepts and Challenges in the Biophysics of Hearing, MoH 2008
Y2 - 27 July 2008 through 31 July 2008
ER -