TY - JOUR
T1 - Trans-suppression of host CDH3 and LOXL4 genes during Cryptosporidium parvum infection involves nuclear delivery of parasite Cdg7_FLc_1000 RNA
AU - Ming, Zhenping
AU - Gong, Ai Yu
AU - Wang, Yang
AU - Zhang, Xin Tian
AU - Li, Min
AU - Li, Yao
AU - Pang, Jing
AU - Dong, Stephanie
AU - Strauss-Soukup, Juliane K.
AU - Chen, Xian Ming
N1 - Funding Information:
We thank Dr. Quanghui Zhao (Northwest A&F University, China) for helpful and stimulating discussions, and Barbara L. Bittner (Creighton University, USA) for her assistance in writing the manuscript. This work was supported by funding from the National Institutes of Health, USA (AI116323 and AI136877) and by revenue from the State of Nebraska, USA, excise tax on cigarettes awarded to Creighton University through the Nebraska Department of Health & Human Services (DHHS) (LB595). Dr. Zhenping Ming was a visiting scholar supported by the China Scholarship Council and the National Natural Science Foundation of China (NSFC No. 31372194). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, DHHS or NSFC.
Funding Information:
This work was supported by the National Institutes of Health (AI116323 and AI136877) and by the revenue from Nebraska’s excise tax on cigarettes awarded to Creighton University through the Nebraska Department of Health & Human Services (DHHS) (LB595) (to X. M. C.).
Funding Information:
We thank Dr. Quanghui Zhao (Northwest A&F University, China) for helpful and stimulating discussions, and Barbara L. Bittner (Creighton University, USA) for her assistance in writing the manuscript. This work was supported by funding from the National Institutes of Health , USA ( AI116323 and AI136877 ) and by revenue from the State of Nebraska, USA, excise tax on cigarettes awarded to Creighton University through the Nebraska Department of Health & Human Services ( DHHS ) ( LB595 ). Dr. Zhenping Ming was a visiting scholar supported by the China Scholarship Council and the National Natural Science Foundation of China (NSFC No. 31372194 ). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, DHHS or NSFC.
Publisher Copyright:
© 2018 Australian Society for Parasitology
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/5
Y1 - 2018/5
N2 - Intestinal infection by Cryptosporidium parvum causes significant alterations in the gene expression profile in host epithelial cells. Previous studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected host cells and may modulate host gene transcription. Using in vitro models of human intestinal cryptosporidiosis, we report here that trans-suppression of the cadherin 3 (CDH3) and lysyl oxidase like 4 (LOXL4) genes in human intestinal epithelial cells following C. parvum infection involves host delivery of the Cdg7_FLc_1000 RNA, a C. parvum RNA that has been previously demonstrated to be delivered into the nuclei of infected host cells. Downregulation of CDH3 and LOXL4 genes was detected in host epithelial cells following C. parvum infection or in cells expressing the parasite Cdg7_FLc_1000 RNA. Knockdown of Cdg7_FLc_1000 attenuated the trans-suppression of CDH3 and LOXL4 genes in host cells induced by infection. Interestingly, Cdg7_FLc_1000 was detected to be recruited to the promoter regions of both CDH3 and LOXL4 gene loci in host cells following C. parvum infection. Host delivery of Cdg7_FLc_1000 promoted the PH domain zinc finger protein 1 (PRDM1)-mediated H3K9 methylation associated with trans-suppression in the CDH3 gene locus, but not the LOXL4 gene. Therefore, our data suggest that host delivery of Cdg7_FLc_1000 causes CDH3 trans-suppression in human intestinal epithelial cells following C. parvum infection through PRDM1-mediated H3K9 methylation in the CDH3 gene locus, whereas Cdg7_FLc_1000 induces trans-suppression of the host LOXL4 gene through H3K9/H3K27 methylation-independent mechanisms.
AB - Intestinal infection by Cryptosporidium parvum causes significant alterations in the gene expression profile in host epithelial cells. Previous studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected host cells and may modulate host gene transcription. Using in vitro models of human intestinal cryptosporidiosis, we report here that trans-suppression of the cadherin 3 (CDH3) and lysyl oxidase like 4 (LOXL4) genes in human intestinal epithelial cells following C. parvum infection involves host delivery of the Cdg7_FLc_1000 RNA, a C. parvum RNA that has been previously demonstrated to be delivered into the nuclei of infected host cells. Downregulation of CDH3 and LOXL4 genes was detected in host epithelial cells following C. parvum infection or in cells expressing the parasite Cdg7_FLc_1000 RNA. Knockdown of Cdg7_FLc_1000 attenuated the trans-suppression of CDH3 and LOXL4 genes in host cells induced by infection. Interestingly, Cdg7_FLc_1000 was detected to be recruited to the promoter regions of both CDH3 and LOXL4 gene loci in host cells following C. parvum infection. Host delivery of Cdg7_FLc_1000 promoted the PH domain zinc finger protein 1 (PRDM1)-mediated H3K9 methylation associated with trans-suppression in the CDH3 gene locus, but not the LOXL4 gene. Therefore, our data suggest that host delivery of Cdg7_FLc_1000 causes CDH3 trans-suppression in human intestinal epithelial cells following C. parvum infection through PRDM1-mediated H3K9 methylation in the CDH3 gene locus, whereas Cdg7_FLc_1000 induces trans-suppression of the host LOXL4 gene through H3K9/H3K27 methylation-independent mechanisms.
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U2 - 10.1016/j.ijpara.2017.10.008
DO - 10.1016/j.ijpara.2017.10.008
M3 - Article
C2 - 29438669
AN - SCOPUS:85042146382
VL - 48
SP - 423
EP - 431
JO - International Journal for Parasitology
JF - International Journal for Parasitology
SN - 0020-7519
IS - 6
ER -