Transduction of staphylococcal cassette chromosome mec elements between strains of Staphylococcus aureus

Caitlyn R. Scharn, Fred C. Tenover, Richard V. Goering

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a well-known public health concern. However, the means by which methicillin resistance genes are transferred among staphylococci in nature remains unknown. Older scientific literature suggests transduction as a means of mecA transfer, but the optimal conditions are reported to require plasmids and potentially a lysogenic phage. These reports preceded discovery of the staphylococcal cassette chromosome mec (SCCmec) elements. We undertook studies to confirm and clarify the conditions promoting transduction of SCCmec in S. aureus populations using well-characterized donor and recipient strains primarily of the USA300 lineage. Both bacteriophages 80α and 29 were capable of transducing SCCmec type IV and SCCmec type I to recipient strains of S. aureus. Pulsed-field gel electrophoresis and mec-associated dru typing were used to confirm the identity of the transductants. Transfer of mecA via transduction occurred at low frequency and required extended selection times for mecA gene expression and the presence of a penicillinase plasmid in the recipient. However, interference with the process by clavulanic acid and the necessity of lysogeny with φ11 in the recipient or the presence of a small (4-kb) tetracycline resistance plasmid, as previously reported, were not confirmed. SCCmec transduction was occasionally associated with substantial deletions or truncation of SCCmec and the arginine catabolic metabolic element in USA300 recipients. Overall, these data clarify the conditions required for SCCmec transduction and document that rearrangements may occur during the process.

Original languageEnglish
Pages (from-to)5233-5238
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume57
Issue number11
DOIs
StatePublished - Nov 2013

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Staphylococcus aureus
Chromosomes
Plasmids
Bacteriophages
Lysogeny
Literature
Penicillinase
Tetracycline Resistance
Clavulanic Acid
Methicillin Resistance
Pulsed Field Gel Electrophoresis
Methicillin-Resistant Staphylococcus aureus
Staphylococcus
Arginine
Public Health
Gene Expression
Population
Genes

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Transduction of staphylococcal cassette chromosome mec elements between strains of Staphylococcus aureus. / Scharn, Caitlyn R.; Tenover, Fred C.; Goering, Richard V.

In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 11, 11.2013, p. 5233-5238.

Research output: Contribution to journalArticle

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