Transmembrane Ca2+ gradient is essential for high anion transport activity of human erythrocytes

Yaping Tu, C. Feng, H. Xu, Z. Y. Guang, Q. W. Lu, F. Y. Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The role of a transmembrane Ca2+ gradient in anion transport by Band 3 of human resealed erythrocyte ghosts has been studied. The results show that a transmembrane Ca2+ gradient is essential for the conformation of erythrocyte Band 3 with higher anion transport activity. The dissipation of the transmembrane Ca2+ gradient by the ionophore A23187 inhibits the anion transport activity. The extent of this inhibition approaches 90% as the Ca2+ concentration on both sides of the ghost membrane is increased to 1.0mM and half-maximum inhibition is observed at 0.25mM Ca2+ Addition of ATP (0.4mM) to the resealing medium can partly reestablish the transmembrane Ca2+ gradient by activation of Ca2+-ATPase and alleviate the inhibition to some extent. N-ethylmaleimide, an inhibitor of erythrocyte Ca2+-ATPase, prevents such restoration. Electron micrographs reveal that numerous larger intramembranous particles can be observed on the P-faces of freeze-fractured resealed ghosts in the absence of a transmembrane Ca2+ gradient.

Original languageEnglish
Pages (from-to)299-311
Number of pages13
JournalBioscience Reports
Volume16
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

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Human Activities
Anions
Calcium-Transporting ATPases
Erythrocytes
Ethylmaleimide
Ionophores
Calcimycin
Erythrocyte Membrane
Restoration
Conformations
Adenosine Triphosphate
Chemical activation
Electrons
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Transmembrane Ca2+ gradient is essential for high anion transport activity of human erythrocytes. / Tu, Yaping; Feng, C.; Xu, H.; Guang, Z. Y.; Lu, Q. W.; Yang, F. Y.

In: Bioscience Reports, Vol. 16, No. 4, 1996, p. 299-311.

Research output: Contribution to journalArticle

Tu, Yaping ; Feng, C. ; Xu, H. ; Guang, Z. Y. ; Lu, Q. W. ; Yang, F. Y. / Transmembrane Ca2+ gradient is essential for high anion transport activity of human erythrocytes. In: Bioscience Reports. 1996 ; Vol. 16, No. 4. pp. 299-311.
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