Transmembrane Ca2+ gradient-mediated change of fluidity in the inner layer of phospholipids modulates Ca2+-ATPase of sarcoplasmic reticulum

Y. P. Tu, H. Xu, F. Y. Yang

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Sarcoplasmic reticulum (SR) vesicles with (1000 folds) or without transmembrane Ca2+ gradient have been prepared. Different fluorescence probes (DPH, TMA-DPH and n-AS), were used to determine the effect of transmembrane Ca2+ gradient on the lipid fluidity both in outer and inner layer of Ca2+-ATPase-containing SR vesicles. The results showed that transmembrane Ca2+ gradient could significantly decrease the fluidity of the inner layer of SR membrane, while no obvious change was monitored in the outer layer. This may be deduced that Ca2+-ATPase might be modulated mainly by the transmembrane Ca2+ gradient-mediated alteration of physical state of phospholipid in the inner layer of SR membrane.

Original languageEnglish (US)
Pages (from-to)597-605
Number of pages9
JournalBiochemistry and Molecular Biology International
Issue number3
StatePublished - Jan 1 1994
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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