Transmissible mink encephalopathy species barrier effect between ferret and mink

PrP gene and protein analysis

Jason C. Bartz, D. I. McKenzie, R. A. Bessen, R. F. Marsh, J. M. Aiken

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Experimental infection of transmissible mink encephalopathy (TME) in two closely related mustelids, black ferret (Mustela putorius furo) and mink (Mustela visa), revealed differences in their susceptibility to the TME agent. When challenged with the Stetsonville TME agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). Western blot analysis of ferret and mink prion proteins (PrP) demonstrated no detectable differences between the proteins. Northern blot analysis of ferret brain RNA indicated that PrP mRNA abundance is similar in infected and uninfected individuals. We amplified the PrP coding region from ferret DNA using the polymerase chain reaction and compared the deduced amino acid sequence of the ferret PrP gene with the mink PrP gene. This comparison revealed six silent base changes and two amino acid changes between mink and ferret: Phe → Lys at codon 179 and Arg → Gln at codon 224, respectively. These changes may indicate the region of PrP that is responsible for the species barrier effect between mink and ferret.

Original languageEnglish
Pages (from-to)2947-2953
Number of pages7
JournalJournal of General Virology
Volume75
Issue number11
StatePublished - 1994
Externally publishedYes

Fingerprint

Mink
Ferrets
Brain Diseases
Proteins
Codon
Prion Proteins
DNA-Directed DNA Polymerase
Northern Blotting
Open Reading Frames
Genes
Amino Acid Sequence
Western Blotting
RNA
Amino Acids
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Transmissible mink encephalopathy species barrier effect between ferret and mink : PrP gene and protein analysis. / Bartz, Jason C.; McKenzie, D. I.; Bessen, R. A.; Marsh, R. F.; Aiken, J. M.

In: Journal of General Virology, Vol. 75, No. 11, 1994, p. 2947-2953.

Research output: Contribution to journalArticle

Bartz, Jason C. ; McKenzie, D. I. ; Bessen, R. A. ; Marsh, R. F. ; Aiken, J. M. / Transmissible mink encephalopathy species barrier effect between ferret and mink : PrP gene and protein analysis. In: Journal of General Virology. 1994 ; Vol. 75, No. 11. pp. 2947-2953.
@article{256b993ae01448919c1efd6140c74bad,
title = "Transmissible mink encephalopathy species barrier effect between ferret and mink: PrP gene and protein analysis",
abstract = "Experimental infection of transmissible mink encephalopathy (TME) in two closely related mustelids, black ferret (Mustela putorius furo) and mink (Mustela visa), revealed differences in their susceptibility to the TME agent. When challenged with the Stetsonville TME agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). Western blot analysis of ferret and mink prion proteins (PrP) demonstrated no detectable differences between the proteins. Northern blot analysis of ferret brain RNA indicated that PrP mRNA abundance is similar in infected and uninfected individuals. We amplified the PrP coding region from ferret DNA using the polymerase chain reaction and compared the deduced amino acid sequence of the ferret PrP gene with the mink PrP gene. This comparison revealed six silent base changes and two amino acid changes between mink and ferret: Phe → Lys at codon 179 and Arg → Gln at codon 224, respectively. These changes may indicate the region of PrP that is responsible for the species barrier effect between mink and ferret.",
author = "Bartz, {Jason C.} and McKenzie, {D. I.} and Bessen, {R. A.} and Marsh, {R. F.} and Aiken, {J. M.}",
year = "1994",
language = "English",
volume = "75",
pages = "2947--2953",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "11",

}

TY - JOUR

T1 - Transmissible mink encephalopathy species barrier effect between ferret and mink

T2 - PrP gene and protein analysis

AU - Bartz, Jason C.

AU - McKenzie, D. I.

AU - Bessen, R. A.

AU - Marsh, R. F.

AU - Aiken, J. M.

PY - 1994

Y1 - 1994

N2 - Experimental infection of transmissible mink encephalopathy (TME) in two closely related mustelids, black ferret (Mustela putorius furo) and mink (Mustela visa), revealed differences in their susceptibility to the TME agent. When challenged with the Stetsonville TME agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). Western blot analysis of ferret and mink prion proteins (PrP) demonstrated no detectable differences between the proteins. Northern blot analysis of ferret brain RNA indicated that PrP mRNA abundance is similar in infected and uninfected individuals. We amplified the PrP coding region from ferret DNA using the polymerase chain reaction and compared the deduced amino acid sequence of the ferret PrP gene with the mink PrP gene. This comparison revealed six silent base changes and two amino acid changes between mink and ferret: Phe → Lys at codon 179 and Arg → Gln at codon 224, respectively. These changes may indicate the region of PrP that is responsible for the species barrier effect between mink and ferret.

AB - Experimental infection of transmissible mink encephalopathy (TME) in two closely related mustelids, black ferret (Mustela putorius furo) and mink (Mustela visa), revealed differences in their susceptibility to the TME agent. When challenged with the Stetsonville TME agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). Western blot analysis of ferret and mink prion proteins (PrP) demonstrated no detectable differences between the proteins. Northern blot analysis of ferret brain RNA indicated that PrP mRNA abundance is similar in infected and uninfected individuals. We amplified the PrP coding region from ferret DNA using the polymerase chain reaction and compared the deduced amino acid sequence of the ferret PrP gene with the mink PrP gene. This comparison revealed six silent base changes and two amino acid changes between mink and ferret: Phe → Lys at codon 179 and Arg → Gln at codon 224, respectively. These changes may indicate the region of PrP that is responsible for the species barrier effect between mink and ferret.

UR - http://www.scopus.com/inward/record.url?scp=0028054270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028054270&partnerID=8YFLogxK

M3 - Article

VL - 75

SP - 2947

EP - 2953

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 11

ER -