TREM-1, HMGB1 and RAGE in the shoulder tendon

Dual mechanisms for inflammation based on the coincidence of glenohumeral arthritis

Finosh G. Thankam, Matthew Dilisio, Nicholas E. Dietz, Devendra K. Agrawal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rotator cuff injury (RCI) is a major musculoskeletal disorder in the adult population where inflammation and pain are major contributing factors. Coincidence of other clinical conditions like glenohumeral arthritis aggravates inflammation and delays the healing response. The mechanism and signaling factors underlying the sustenance of inflammation in the rotator cuff joint are largely unknown. The present article aims to elucidate the involvement of inflammatory molecule, TREM-1 (Triggering Receptors Expressed on Myeloid cells-1), and dangerassociated molecular patterns (DAMPs), including high mobility group protein 1 (HMGB-1) and RAGE (receptor for advanced glycation end products), in the setting of RCI with respect to the severity of glenohumeral arthritis. Biceps tendons (15 specimens) from the shoulder and blood (11 samples) from patients with glenohumeral arthritis (Group-1, n = 4) and without glenohumeral arthritis (Group-2, n = 11) after RCI surgery were obtained for the study. Molecular and morphological alterations between the groups were compared using histology, immunofluorescence, RT-PCR and flow cytometry. MRI and histomorphology assessment revealed severe inflammation in Group-1 patients while in Group-2 ECM disorganization was prominent without any hallmarks of inflammation. A significant increase in TREM-1 expression in circulating neutrophils and monocytes was observed. Elevated levels of TREM-1, HMGB-1 and RAGE in Group-1 patients along with CD68+ and CD16+ cells confirmed DAMP-mediated inflammation. Expression of TREM-1 in the tendon of Group-2 patients even in the absence of immune cells presented a new population of TREM-expressing cells that were confirmed by real-time PCR analysis and immunofluorescence. Expression of HMGB-1 and RAGE in the biceps tendon from the shoulder of patients without glenohumeral arthritis implied TREM-1-mediated inflammation without involving immune cells, whereas in patients with glenohumeral arthritis, infiltration and the activation of the immune cells, primarily macrophages, release mediators to induce inflammation. This could be the reason for ECM disorganization without the classical signs of inflammation in patients without glenohumeral arthritis.

Original languageEnglish (US)
Article numbere0165492
JournalPLoS One
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

HMGB Proteins
HMGB1 Protein
Tendons
arthritis
Myeloid Cells
tendons
shoulders
Arthritis
Military electronic countermeasures
inflammation
Inflammation
receptors
High Mobility Group Proteins
Histology
Flow cytometry
Macrophages
cells
Infiltration
Magnetic resonance imaging
Surgery

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

TREM-1, HMGB1 and RAGE in the shoulder tendon : Dual mechanisms for inflammation based on the coincidence of glenohumeral arthritis. / Thankam, Finosh G.; Dilisio, Matthew; Dietz, Nicholas E.; Agrawal, Devendra K.

In: PLoS One, Vol. 11, No. 10, e0165492, 01.10.2016.

Research output: Contribution to journalArticle

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