Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer

Martin Lipkin, Eleanor Deschner, Sidney Winawer, William E. Blattner, Joseph F. Fraumeni, Henry T. Lynch

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205 Citations (Scopus)

Abstract

New analytical methods have been developed for measurement of the height distribution patterns of [3H]dThd-labeled epithelial cells in colonic crypts of high- and low-risk population groups. Labeled cells were segregated with respect to crypt-height into 10 compartments of equal size, plus a lumenal surface compartment for each subject. Members of families prone to polyposis coli and to non-polyposis colon cancer were compared to subjects at lower risk. The latter included persons from polyp-free and cancer-free branches of the same families, normal controls, and patients with colon cancer from the general population. Significant differences were found between groups of patients with familial polyposis or familial colon cancer and subjects at low risk, when labeled cell distributions were compared over all the crypt height compartments (p <0.001). Distributions of the occupancy fractions of labeled cells in the upper region (i.e. 40%) of the crypt (Φh), measured for the fraction of each population (Φp), revealed a discriminant level that separated over 90% of low-risk subjects from a major fraction of those affected with familial colon cancer or polyposis and from close to one-half of the at-risk progeny as expected for an autosomal dominant trait. However, subjects with colon cancer in the general population had (Φp,Φh) distributions closer to the low-risk groups, although a subgroup of patients had abnormal (Φh), values characteristic of hereditary disease. Thus, the (Φp,Φh) distribution appears to be a more precise measure of risk than previously used in discriminating populations with genetic susceptibility to colon cancer from those at lower risk and may be useful as a marker to identify individuals with the at-risk phenotype.

Original languageEnglish
Pages (from-to)1899-1904
Number of pages6
JournalCancer Research
Volume43
Issue number4
StatePublished - Apr 1 1983

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Colonic Neoplasms
Thymidine
Population
Adenomatous Polyposis Coli
Demography
Inborn Genetic Diseases
Population Genetics
Genetic Predisposition to Disease
Polyps
Population Groups
Epithelial Cells
Phenotype

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Lipkin, M., Deschner, E., Winawer, S., Blattner, W. E., Fraumeni, J. F., & Lynch, H. T. (1983). Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer. Cancer Research, 43(4), 1899-1904.

Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer. / Lipkin, Martin; Deschner, Eleanor; Winawer, Sidney; Blattner, William E.; Fraumeni, Joseph F.; Lynch, Henry T.

In: Cancer Research, Vol. 43, No. 4, 01.04.1983, p. 1899-1904.

Research output: Contribution to journalArticle

Lipkin, M, Deschner, E, Winawer, S, Blattner, WE, Fraumeni, JF & Lynch, HT 1983, 'Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer', Cancer Research, vol. 43, no. 4, pp. 1899-1904.
Lipkin M, Deschner E, Winawer S, Blattner WE, Fraumeni JF, Lynch HT. Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer. Cancer Research. 1983 Apr 1;43(4):1899-1904.
Lipkin, Martin ; Deschner, Eleanor ; Winawer, Sidney ; Blattner, William E. ; Fraumeni, Joseph F. ; Lynch, Henry T. / Tritiated Thymidine (Φp,Φh) Labeling Distribution as a Marker for hereditary predisposition to colon cancer. In: Cancer Research. 1983 ; Vol. 43, No. 4. pp. 1899-1904.
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abstract = "New analytical methods have been developed for measurement of the height distribution patterns of [3H]dThd-labeled epithelial cells in colonic crypts of high- and low-risk population groups. Labeled cells were segregated with respect to crypt-height into 10 compartments of equal size, plus a lumenal surface compartment for each subject. Members of families prone to polyposis coli and to non-polyposis colon cancer were compared to subjects at lower risk. The latter included persons from polyp-free and cancer-free branches of the same families, normal controls, and patients with colon cancer from the general population. Significant differences were found between groups of patients with familial polyposis or familial colon cancer and subjects at low risk, when labeled cell distributions were compared over all the crypt height compartments (p <0.001). Distributions of the occupancy fractions of labeled cells in the upper region (i.e. 40{\%}) of the crypt (Φh), measured for the fraction of each population (Φp), revealed a discriminant level that separated over 90{\%} of low-risk subjects from a major fraction of those affected with familial colon cancer or polyposis and from close to one-half of the at-risk progeny as expected for an autosomal dominant trait. However, subjects with colon cancer in the general population had (Φp,Φh) distributions closer to the low-risk groups, although a subgroup of patients had abnormal (Φh), values characteristic of hereditary disease. Thus, the (Φp,Φh) distribution appears to be a more precise measure of risk than previously used in discriminating populations with genetic susceptibility to colon cancer from those at lower risk and may be useful as a marker to identify individuals with the at-risk phenotype.",
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