TY - JOUR
T1 - Tumor necrosis factor-α hyper-responsiveness to endotoxin in whole blood is associated with chronic bronchitis in farmers
AU - Lambert, G. Patrick
AU - Spurzem, John R.
AU - Romberger, Debra J.
AU - Wyatt, Todd A.
AU - Lyden, Elizabeth
AU - Stromquist, Ann M.
AU - Merchant, James A.
AU - Von Essen, Susanna G.
N1 - Funding Information:
This research was supported (in part) by a pilot project research training grant from the Heartland Center for Occupational Health and Safety at the University of Iowa to Dr. Lambert. The Heartland Center, an Education and Research Center, is supported by Training Grant No. T42/CCT 717547 from the Centers for Disease Control and Prevention/National Institute for Occupational Safety and Health. The contents are solely the responsibility of the author(s) and do not necessarily represent the official views of the National Institute for Occupational Safety and Health.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Many farmers experience chronic bronchitis, airflow obstruction, and asthma. It is thought that these respiratory problems may be related to workplace inhalation of organic dust containing endotoxin. The purpose of this study was to determine whether whole blood cytokine responsiveness to endotoxin is associated with airflow disorders (i.e., airflow obstruction, chronic bronchitis, and doctor-diagnosed asthma). Farmers (N = 95) were recruited from a rural cohort study and completed a respiratory symptom and history questionnaire, spirometry, and blood sampling. Blood was incubated 24 hours in the presence and absence of endotoxin and supernatants were analyzed for TNF-α, IL-1ß, IL-6, and IL-8. Hypo- or hyper-responsiveness to endotoxin was based on whether cytokine values were in the lower or upper 10% of the group range, respectively. A significant association existed between TNF-α hyper-responsiveness and chronic bronchitis. These results indicate that the whole blood cytokine assay may be useful to identify individual responsiveness to endotoxin, and may provide an additional diagnostic tool to evaluate persons potentially at risk for developing chronic bronchitis following exposure to organic dust in the workplace.
AB - Many farmers experience chronic bronchitis, airflow obstruction, and asthma. It is thought that these respiratory problems may be related to workplace inhalation of organic dust containing endotoxin. The purpose of this study was to determine whether whole blood cytokine responsiveness to endotoxin is associated with airflow disorders (i.e., airflow obstruction, chronic bronchitis, and doctor-diagnosed asthma). Farmers (N = 95) were recruited from a rural cohort study and completed a respiratory symptom and history questionnaire, spirometry, and blood sampling. Blood was incubated 24 hours in the presence and absence of endotoxin and supernatants were analyzed for TNF-α, IL-1ß, IL-6, and IL-8. Hypo- or hyper-responsiveness to endotoxin was based on whether cytokine values were in the lower or upper 10% of the group range, respectively. A significant association existed between TNF-α hyper-responsiveness and chronic bronchitis. These results indicate that the whole blood cytokine assay may be useful to identify individual responsiveness to endotoxin, and may provide an additional diagnostic tool to evaluate persons potentially at risk for developing chronic bronchitis following exposure to organic dust in the workplace.
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U2 - 10.1300/J096v10n01_05
DO - 10.1300/J096v10n01_05
M3 - Article
C2 - 15927916
AN - SCOPUS:24044507009
VL - 10
SP - 39
EP - 44
JO - Journal of Agromedicine
JF - Journal of Agromedicine
SN - 1059-924X
IS - 1
ER -