University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts

Nicola Cavallari, Worku Abebe, William J. Hunter, Devendra K. Agrawal, Paolo Sapienza, Andrea Mingoli, Antonino Cavallaro, John D. Edwards

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study was carried out to investigate if the University of Wisconsin solution (UWs) is suitable for long-term preservation of autogenous vein grafts (AVG) prior to transplantation, compared to autologous whole blood (AWB) and normal saline (NS). Autogenous jugular and femoral veins were harvested from adult mongrel dogs, using a "no-touch" technique. One segment of vein was immediately implanted to serve as a control, while other segments were stored for 24 hr at 4°C in AWB, NS, or UWs. The control and the preserved veins were implanted as reversed interposition grafts in the common carotid or femoral artery positions. After 6 weeks, scanning electron microscopy revealed an intact endothelial cell monolayer in all vein grafts that was stained positively for factor VIII. The degree of intimal thickening, as assessed by light microscopy in the middle position of the grafts, was similar in controls (52.0 ± 15.8 μm) and in veins stored in UWs (58.5 ± 16.1 μm), but it was significantly increased (P <0.05) in veins preserved in NS (198.9 ± 19.5 μm) and in AWB (312.0 ± 171.6 μm). Isometric tension studies revealed that maximum contraction and sensitivities (assessed by EC50) to norepinephrine were significantly reduced (P <0.05) in AVG stored in AWB (0.05 ± 0.02 g/mm2 and 5.5 ± 2.8 μM), but not in UWs (0.16 ± 0.03 g/mm2 0.92 ± 0.34 ±M) and NS (0.09 ± 0.03 g/mm2 and 3.0 ± 1.1 μM), compared with controls (0.17 ± 0.03 g/mm2 and 0.99 ± 0.38 μM). Acetylcholine (Ach)-induced maximum relaxations were similar in all of the veins. However, vessels stored in AWB were found to be more sensitive (P <0.05) to Ach (0.004 ± 0.002 μM) than those stored in UWs (0.042 ± 0.018 μM). Sodium nitroprusside produced similar responses in all of the veins. These data demonstrated that storage of veins in UWs for 24 hr prior to transplantation is more suitable than storage in AWB and NS for preserving the morphology and functional integrity of the smooth muscle and endothelium.

Original languageEnglish
Pages (from-to)433-440
Number of pages8
JournalJournal of Surgical Research
Volume59
Issue number4
DOIs
StatePublished - Oct 1995

Fingerprint

Tunica Intima
Canidae
Veins
Transplants
Acetylcholine
Transplantation
Femoral Vein
Information Storage and Retrieval
Common Carotid Artery
Jugular Veins
Factor VIII
Touch
Nitroprusside
Femoral Artery
Electron Scanning Microscopy
Endothelium
Smooth Muscle
Microscopy
Norepinephrine
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts. / Cavallari, Nicola; Abebe, Worku; Hunter, William J.; Agrawal, Devendra K.; Sapienza, Paolo; Mingoli, Andrea; Cavallaro, Antonino; Edwards, John D.

In: Journal of Surgical Research, Vol. 59, No. 4, 10.1995, p. 433-440.

Research output: Contribution to journalArticle

Cavallari, N, Abebe, W, Hunter, WJ, Agrawal, DK, Sapienza, P, Mingoli, A, Cavallaro, A & Edwards, JD 1995, 'University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts', Journal of Surgical Research, vol. 59, no. 4, pp. 433-440. https://doi.org/10.1006/jsre.1995.1187
Cavallari, Nicola ; Abebe, Worku ; Hunter, William J. ; Agrawal, Devendra K. ; Sapienza, Paolo ; Mingoli, Andrea ; Cavallaro, Antonino ; Edwards, John D. / University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts. In: Journal of Surgical Research. 1995 ; Vol. 59, No. 4. pp. 433-440.
@article{b235b9058ce041f285142e129415160d,
title = "University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts",
abstract = "This study was carried out to investigate if the University of Wisconsin solution (UWs) is suitable for long-term preservation of autogenous vein grafts (AVG) prior to transplantation, compared to autologous whole blood (AWB) and normal saline (NS). Autogenous jugular and femoral veins were harvested from adult mongrel dogs, using a {"}no-touch{"} technique. One segment of vein was immediately implanted to serve as a control, while other segments were stored for 24 hr at 4°C in AWB, NS, or UWs. The control and the preserved veins were implanted as reversed interposition grafts in the common carotid or femoral artery positions. After 6 weeks, scanning electron microscopy revealed an intact endothelial cell monolayer in all vein grafts that was stained positively for factor VIII. The degree of intimal thickening, as assessed by light microscopy in the middle position of the grafts, was similar in controls (52.0 ± 15.8 μm) and in veins stored in UWs (58.5 ± 16.1 μm), but it was significantly increased (P <0.05) in veins preserved in NS (198.9 ± 19.5 μm) and in AWB (312.0 ± 171.6 μm). Isometric tension studies revealed that maximum contraction and sensitivities (assessed by EC50) to norepinephrine were significantly reduced (P <0.05) in AVG stored in AWB (0.05 ± 0.02 g/mm2 and 5.5 ± 2.8 μM), but not in UWs (0.16 ± 0.03 g/mm2 0.92 ± 0.34 ±M) and NS (0.09 ± 0.03 g/mm2 and 3.0 ± 1.1 μM), compared with controls (0.17 ± 0.03 g/mm2 and 0.99 ± 0.38 μM). Acetylcholine (Ach)-induced maximum relaxations were similar in all of the veins. However, vessels stored in AWB were found to be more sensitive (P <0.05) to Ach (0.004 ± 0.002 μM) than those stored in UWs (0.042 ± 0.018 μM). Sodium nitroprusside produced similar responses in all of the veins. These data demonstrated that storage of veins in UWs for 24 hr prior to transplantation is more suitable than storage in AWB and NS for preserving the morphology and functional integrity of the smooth muscle and endothelium.",
author = "Nicola Cavallari and Worku Abebe and Hunter, {William J.} and Agrawal, {Devendra K.} and Paolo Sapienza and Andrea Mingoli and Antonino Cavallaro and Edwards, {John D.}",
year = "1995",
month = "10",
doi = "10.1006/jsre.1995.1187",
language = "English",
volume = "59",
pages = "433--440",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - University of Wisconsin Solution Effects on Intimal Proliferation in Canine Autogenous Vein Grafts

AU - Cavallari, Nicola

AU - Abebe, Worku

AU - Hunter, William J.

AU - Agrawal, Devendra K.

AU - Sapienza, Paolo

AU - Mingoli, Andrea

AU - Cavallaro, Antonino

AU - Edwards, John D.

PY - 1995/10

Y1 - 1995/10

N2 - This study was carried out to investigate if the University of Wisconsin solution (UWs) is suitable for long-term preservation of autogenous vein grafts (AVG) prior to transplantation, compared to autologous whole blood (AWB) and normal saline (NS). Autogenous jugular and femoral veins were harvested from adult mongrel dogs, using a "no-touch" technique. One segment of vein was immediately implanted to serve as a control, while other segments were stored for 24 hr at 4°C in AWB, NS, or UWs. The control and the preserved veins were implanted as reversed interposition grafts in the common carotid or femoral artery positions. After 6 weeks, scanning electron microscopy revealed an intact endothelial cell monolayer in all vein grafts that was stained positively for factor VIII. The degree of intimal thickening, as assessed by light microscopy in the middle position of the grafts, was similar in controls (52.0 ± 15.8 μm) and in veins stored in UWs (58.5 ± 16.1 μm), but it was significantly increased (P <0.05) in veins preserved in NS (198.9 ± 19.5 μm) and in AWB (312.0 ± 171.6 μm). Isometric tension studies revealed that maximum contraction and sensitivities (assessed by EC50) to norepinephrine were significantly reduced (P <0.05) in AVG stored in AWB (0.05 ± 0.02 g/mm2 and 5.5 ± 2.8 μM), but not in UWs (0.16 ± 0.03 g/mm2 0.92 ± 0.34 ±M) and NS (0.09 ± 0.03 g/mm2 and 3.0 ± 1.1 μM), compared with controls (0.17 ± 0.03 g/mm2 and 0.99 ± 0.38 μM). Acetylcholine (Ach)-induced maximum relaxations were similar in all of the veins. However, vessels stored in AWB were found to be more sensitive (P <0.05) to Ach (0.004 ± 0.002 μM) than those stored in UWs (0.042 ± 0.018 μM). Sodium nitroprusside produced similar responses in all of the veins. These data demonstrated that storage of veins in UWs for 24 hr prior to transplantation is more suitable than storage in AWB and NS for preserving the morphology and functional integrity of the smooth muscle and endothelium.

AB - This study was carried out to investigate if the University of Wisconsin solution (UWs) is suitable for long-term preservation of autogenous vein grafts (AVG) prior to transplantation, compared to autologous whole blood (AWB) and normal saline (NS). Autogenous jugular and femoral veins were harvested from adult mongrel dogs, using a "no-touch" technique. One segment of vein was immediately implanted to serve as a control, while other segments were stored for 24 hr at 4°C in AWB, NS, or UWs. The control and the preserved veins were implanted as reversed interposition grafts in the common carotid or femoral artery positions. After 6 weeks, scanning electron microscopy revealed an intact endothelial cell monolayer in all vein grafts that was stained positively for factor VIII. The degree of intimal thickening, as assessed by light microscopy in the middle position of the grafts, was similar in controls (52.0 ± 15.8 μm) and in veins stored in UWs (58.5 ± 16.1 μm), but it was significantly increased (P <0.05) in veins preserved in NS (198.9 ± 19.5 μm) and in AWB (312.0 ± 171.6 μm). Isometric tension studies revealed that maximum contraction and sensitivities (assessed by EC50) to norepinephrine were significantly reduced (P <0.05) in AVG stored in AWB (0.05 ± 0.02 g/mm2 and 5.5 ± 2.8 μM), but not in UWs (0.16 ± 0.03 g/mm2 0.92 ± 0.34 ±M) and NS (0.09 ± 0.03 g/mm2 and 3.0 ± 1.1 μM), compared with controls (0.17 ± 0.03 g/mm2 and 0.99 ± 0.38 μM). Acetylcholine (Ach)-induced maximum relaxations were similar in all of the veins. However, vessels stored in AWB were found to be more sensitive (P <0.05) to Ach (0.004 ± 0.002 μM) than those stored in UWs (0.042 ± 0.018 μM). Sodium nitroprusside produced similar responses in all of the veins. These data demonstrated that storage of veins in UWs for 24 hr prior to transplantation is more suitable than storage in AWB and NS for preserving the morphology and functional integrity of the smooth muscle and endothelium.

UR - http://www.scopus.com/inward/record.url?scp=0028871729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028871729&partnerID=8YFLogxK

U2 - 10.1006/jsre.1995.1187

DO - 10.1006/jsre.1995.1187

M3 - Article

VL - 59

SP - 433

EP - 440

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 4

ER -