Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis

J. Qin, Y. Xie, B. Wang, M. Hoshino, D. W. Wolff, J. Zhao, M. A. Scofield, F. J. Dowd, M. F. Lin, Yaping Tu

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Excessive activation of G-protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) pathways has been linked to prostate cancer metastasis. Rac activation by guanine nucleotide exchange factors (GEFs) plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We found that the upregulation of P-Rex1, a Rac-selective GEF synergistically activated by Gβγ freed during GPCR signaling, and PIP3, generated during either RTK or GPCR signaling, strongly correlates with metastatic phenotypes in both prostate cancer cell lines and human prostate cancer specimens. Silencing endogenous P-Rex1 in metastatic prostate cancer PC-3 cells selectively inhibited Rac activity and reduced cell migration and invasion in response to ligands of both epidermal growth factor receptor and G-protein-coupled CXC chemokine receptor 4. Conversely, expression of recombinant P-Rex1, but not its GEF-dead mutant, in non-metastatic prostate cancer cells, such as CWR22Rv1, increased cell migration and invasion through Rac-dependent lamellipodia formation. More importantly, using a mouse xenograft model, we showed that the expression of P-Rex1, but not its mutant, induced lymph node metastasis of CWR22Rv1 cells without an effect on primary tumor growth. Thus, by functioning as a coincidence detector of chemotactic signals from both GPCRs and RTKs, P-Rex1-dependent activation of Rac promotes prostate cancer metastasis.Oncogene (2009).

Original languageEnglish
Pages (from-to)1853-1863
Number of pages11
JournalOncogene
Volume28
Issue number16
DOIs
StatePublished - Apr 23 2009

Fingerprint

Prostatic Neoplasms
Up-Regulation
Neoplasm Metastasis
Guanine Nucleotide Exchange Factors
Cell Movement
G-Protein-Coupled Receptors
G-Protein-Coupled Receptor Kinases
CXCR4 Receptors
Pseudopodia
Receptor Protein-Tyrosine Kinases
Oncogenes
GTP-Binding Proteins
Epidermal Growth Factor Receptor
Heterografts
Neoplasms
Lymph Nodes
Ligands
Phenotype
Cell Line
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis. / Qin, J.; Xie, Y.; Wang, B.; Hoshino, M.; Wolff, D. W.; Zhao, J.; Scofield, M. A.; Dowd, F. J.; Lin, M. F.; Tu, Yaping.

In: Oncogene, Vol. 28, No. 16, 23.04.2009, p. 1853-1863.

Research output: Contribution to journalArticle

Qin, J, Xie, Y, Wang, B, Hoshino, M, Wolff, DW, Zhao, J, Scofield, MA, Dowd, FJ, Lin, MF & Tu, Y 2009, 'Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis', Oncogene, vol. 28, no. 16, pp. 1853-1863. https://doi.org/10.1038/onc.2009.30
Qin J, Xie Y, Wang B, Hoshino M, Wolff DW, Zhao J et al. Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis. Oncogene. 2009 Apr 23;28(16):1853-1863. https://doi.org/10.1038/onc.2009.30
Qin, J. ; Xie, Y. ; Wang, B. ; Hoshino, M. ; Wolff, D. W. ; Zhao, J. ; Scofield, M. A. ; Dowd, F. J. ; Lin, M. F. ; Tu, Yaping. / Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis. In: Oncogene. 2009 ; Vol. 28, No. 16. pp. 1853-1863.
@article{2faa994a5d2d4511ab433aec5d2dbbbd,
title = "Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis",
abstract = "Excessive activation of G-protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) pathways has been linked to prostate cancer metastasis. Rac activation by guanine nucleotide exchange factors (GEFs) plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We found that the upregulation of P-Rex1, a Rac-selective GEF synergistically activated by Gβγ freed during GPCR signaling, and PIP3, generated during either RTK or GPCR signaling, strongly correlates with metastatic phenotypes in both prostate cancer cell lines and human prostate cancer specimens. Silencing endogenous P-Rex1 in metastatic prostate cancer PC-3 cells selectively inhibited Rac activity and reduced cell migration and invasion in response to ligands of both epidermal growth factor receptor and G-protein-coupled CXC chemokine receptor 4. Conversely, expression of recombinant P-Rex1, but not its GEF-dead mutant, in non-metastatic prostate cancer cells, such as CWR22Rv1, increased cell migration and invasion through Rac-dependent lamellipodia formation. More importantly, using a mouse xenograft model, we showed that the expression of P-Rex1, but not its mutant, induced lymph node metastasis of CWR22Rv1 cells without an effect on primary tumor growth. Thus, by functioning as a coincidence detector of chemotactic signals from both GPCRs and RTKs, P-Rex1-dependent activation of Rac promotes prostate cancer metastasis.Oncogene (2009).",
author = "J. Qin and Y. Xie and B. Wang and M. Hoshino and Wolff, {D. W.} and J. Zhao and Scofield, {M. A.} and Dowd, {F. J.} and Lin, {M. F.} and Yaping Tu",
year = "2009",
month = "4",
day = "23",
doi = "10.1038/onc.2009.30",
language = "English",
volume = "28",
pages = "1853--1863",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "16",

}

TY - JOUR

T1 - Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis

AU - Qin, J.

AU - Xie, Y.

AU - Wang, B.

AU - Hoshino, M.

AU - Wolff, D. W.

AU - Zhao, J.

AU - Scofield, M. A.

AU - Dowd, F. J.

AU - Lin, M. F.

AU - Tu, Yaping

PY - 2009/4/23

Y1 - 2009/4/23

N2 - Excessive activation of G-protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) pathways has been linked to prostate cancer metastasis. Rac activation by guanine nucleotide exchange factors (GEFs) plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We found that the upregulation of P-Rex1, a Rac-selective GEF synergistically activated by Gβγ freed during GPCR signaling, and PIP3, generated during either RTK or GPCR signaling, strongly correlates with metastatic phenotypes in both prostate cancer cell lines and human prostate cancer specimens. Silencing endogenous P-Rex1 in metastatic prostate cancer PC-3 cells selectively inhibited Rac activity and reduced cell migration and invasion in response to ligands of both epidermal growth factor receptor and G-protein-coupled CXC chemokine receptor 4. Conversely, expression of recombinant P-Rex1, but not its GEF-dead mutant, in non-metastatic prostate cancer cells, such as CWR22Rv1, increased cell migration and invasion through Rac-dependent lamellipodia formation. More importantly, using a mouse xenograft model, we showed that the expression of P-Rex1, but not its mutant, induced lymph node metastasis of CWR22Rv1 cells without an effect on primary tumor growth. Thus, by functioning as a coincidence detector of chemotactic signals from both GPCRs and RTKs, P-Rex1-dependent activation of Rac promotes prostate cancer metastasis.Oncogene (2009).

AB - Excessive activation of G-protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) pathways has been linked to prostate cancer metastasis. Rac activation by guanine nucleotide exchange factors (GEFs) plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We found that the upregulation of P-Rex1, a Rac-selective GEF synergistically activated by Gβγ freed during GPCR signaling, and PIP3, generated during either RTK or GPCR signaling, strongly correlates with metastatic phenotypes in both prostate cancer cell lines and human prostate cancer specimens. Silencing endogenous P-Rex1 in metastatic prostate cancer PC-3 cells selectively inhibited Rac activity and reduced cell migration and invasion in response to ligands of both epidermal growth factor receptor and G-protein-coupled CXC chemokine receptor 4. Conversely, expression of recombinant P-Rex1, but not its GEF-dead mutant, in non-metastatic prostate cancer cells, such as CWR22Rv1, increased cell migration and invasion through Rac-dependent lamellipodia formation. More importantly, using a mouse xenograft model, we showed that the expression of P-Rex1, but not its mutant, induced lymph node metastasis of CWR22Rv1 cells without an effect on primary tumor growth. Thus, by functioning as a coincidence detector of chemotactic signals from both GPCRs and RTKs, P-Rex1-dependent activation of Rac promotes prostate cancer metastasis.Oncogene (2009).

UR - http://www.scopus.com/inward/record.url?scp=67349167687&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349167687&partnerID=8YFLogxK

U2 - 10.1038/onc.2009.30

DO - 10.1038/onc.2009.30

M3 - Article

VL - 28

SP - 1853

EP - 1863

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 16

ER -