Use of miRNAs to Study Host Cell–Parasite Interactions

Ji Hao Xu; Nicholas W. Mathy; Xian Ming Chen

doi:10.1007/978-1-4939-9748-0_12

Use of miRNAs to Study Host Cell–Parasite Interactions

Ji Hao Xu, Nicholas W. Mathy, Xian Ming Chen

Department of Medical Microbiology and Immunology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	205-218
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-9748-0_12
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2052
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/978-1-4939-9748-0_12

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abstract = "MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.",

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year = "2020",

doi = "10.1007/978-1-4939-9748-0_12",

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AB - MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Use of miRNAs to Study Host Cell–Parasite Interactions

Abstract

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