Use of miRNAs to Study Host Cell–Parasite Interactions

Ji Hao Xu, Nicholas W. Mathy, Xian Ming Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-218
Number of pages14
DOIs
Publication statusPublished - Jan 1 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Xu, J. H., Mathy, N. W., & Chen, X. M. (2020). Use of miRNAs to Study Host Cell–Parasite Interactions. In Methods in Molecular Biology (pp. 205-218). (Methods in Molecular Biology; Vol. 2052). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9748-0_12