Abstract
MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 205-218 |
| Number of pages | 14 |
| DOIs | |
| State | Published - Jan 1 2020 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2052 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Use of miRNAs to Study Host Cell–Parasite Interactions. / Xu, Ji Hao; Mathy, Nicholas W.; Chen, Xian-Ming.
Methods in Molecular Biology. Humana Press Inc., 2020. p. 205-218 (Methods in Molecular Biology; Vol. 2052).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Use of miRNAs to Study Host Cell–Parasite Interactions
AU - Xu, Ji Hao
AU - Mathy, Nicholas W.
AU - Chen, Xian-Ming
PY - 2020/1/1
Y1 - 2020/1/1
N2 - MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.
AB - MicroRNAs (miRNAs) represent a subclass of endogenous small noncoding RNAs that have been identified in both mammalian and nonmammalian cells. miRNAs are an essential part of the complex regulatory networks that control numerous biological processes and may play an important role in host defense and/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.
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UR - http://www.scopus.com/inward/citedby.url?scp=85071617641&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9748-0_12
DO - 10.1007/978-1-4939-9748-0_12
M3 - Chapter
T3 - Methods in Molecular Biology
SP - 205
EP - 218
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -