V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis

Kristen M. Drescher, Sean L. Johnston, William Hogancamp, Gerald H. Nabozny, Chella S. David, Ilonna J. Rimm, Peter J. Wettstein, Moses Rodriguez

Research output: Contribution to journalArticle

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Abstract

Previous studies illustrated the influence of T cell subsets on susceptibility or resistance to demyelination in the Theiler's murine encephalomyelitis virus (TMEV) model of multiple sclerosis. Genetic segregation analysis showed a correlation with disease phenotype in this model with particular V(β) genes. In this study we investigated the contribution of specific V(β) TCR to the pathogenesis of virus-induced demyelinating disease. Spectratype analysis of cells infiltrating the CNS early in infection demonstrated an over-representation of V(β)8+ T cells in mice expressing a susceptible H-2 haplotype. We infected transgenic mice expressing the V(β)8.2 TCR directed against a non-TMEV antigen and found an increase in demyelinating disease in mice of either susceptible or resistant background compared with littermate controls. In addition, depletion studies with an anti-V(β)8-specific antibody in both susceptible (B10.Q) and resistant (C57BL/6) mice resulted in increased demyelination. TCR analysis of VP2-specific cytotoxic T cell clones from mice with a resistant genotype identified only the V(β)8.1 TCR, suggesting that limited T cell diversity is critical to TMEV clearance. Together, these results support a protective role for V(β)8+ T cells in virus-induced demyelinating disease.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalInternational Immunology
Volume12
Issue number3
StatePublished - 2000

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Demyelinating Diseases
Multiple Sclerosis
T-Lymphocytes
Theilovirus
Maus Elberfeld virus
Viruses
T-Lymphocyte Subsets
Inbred C57BL Mouse
Haplotypes
Transgenic Mice
Clone Cells
Genotype
Phenotype
Antigens
Antibodies
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Drescher, K. M., Johnston, S. L., Hogancamp, W., Nabozny, G. H., David, C. S., Rimm, I. J., ... Rodriguez, M. (2000). V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis. International Immunology, 12(3), 271-280.

V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis. / Drescher, Kristen M.; Johnston, Sean L.; Hogancamp, William; Nabozny, Gerald H.; David, Chella S.; Rimm, Ilonna J.; Wettstein, Peter J.; Rodriguez, Moses.

In: International Immunology, Vol. 12, No. 3, 2000, p. 271-280.

Research output: Contribution to journalArticle

Drescher, KM, Johnston, SL, Hogancamp, W, Nabozny, GH, David, CS, Rimm, IJ, Wettstein, PJ & Rodriguez, M 2000, 'V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis', International Immunology, vol. 12, no. 3, pp. 271-280.
Drescher KM, Johnston SL, Hogancamp W, Nabozny GH, David CS, Rimm IJ et al. V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis. International Immunology. 2000;12(3):271-280.
Drescher, Kristen M. ; Johnston, Sean L. ; Hogancamp, William ; Nabozny, Gerald H. ; David, Chella S. ; Rimm, Ilonna J. ; Wettstein, Peter J. ; Rodriguez, Moses. / V(β)8+ T cells protect from demyelinating disease in a viral model of multiple sclerosis. In: International Immunology. 2000 ; Vol. 12, No. 3. pp. 271-280.
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