Vasopressin receptor mediated contraction and [3H]inositol metabolism in rat tail artery

Anthony W. Fox, Paul A. Friedman, Peter W. Abel

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Inositol phosphates (IP) production and contraction in isolated but otherwise intact rat tail artery were measured in response to stimulation by vasopressin agonists. We have previously studied similar α-adrenoceptor responses. Identical rank orders of vasopressin agonists' potency were found for IP accumulation and contraction. The vasopressin analogue [1-(β-mercapto-β,β-cyclopentamethylene proprionic acid)-2-(O-methyl)tyrosine,D-arginine8] vasopressin, was shown to be a specific, reversible antagonist for both IP accumulation and contraction by all vasopressin agonists tested. No antagonism of vasopressin induced increases in IP accumulation or contraction were found using phenoxybenzamine. Therefore, (i) in this tissue vasopressin receptors mediate both contraction and IP accumulation; and (ii) vasopressin mediated responses appear to be direct effects not mediated via the activation of α-adrenoceptors. Demonstration of two entirely different receptors, mediating the same functional response, and which both promote IP production, is consistent with a general obligatory role for phosphoinositide catabolism in receptor mediated vascular smooth muscle contraction.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalEuropean Journal of Pharmacology
Volume135
Issue number1
DOIs
StatePublished - Mar 3 1987
Externally publishedYes

Fingerprint

Vasopressin Receptors
Inositol
Inositol Phosphates
Vasopressins
Tail
Arteries
Adrenergic Receptors
Phenoxybenzamine
Muscle Contraction
Phosphatidylinositols
Vascular Smooth Muscle
Tyrosine
Acids

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Vasopressin receptor mediated contraction and [3H]inositol metabolism in rat tail artery. / Fox, Anthony W.; Friedman, Paul A.; Abel, Peter W.

In: European Journal of Pharmacology, Vol. 135, No. 1, 03.03.1987, p. 1-10.

Research output: Contribution to journalArticle

@article{45dadca42421470e89370c436a047366,
title = "Vasopressin receptor mediated contraction and [3H]inositol metabolism in rat tail artery",
abstract = "Inositol phosphates (IP) production and contraction in isolated but otherwise intact rat tail artery were measured in response to stimulation by vasopressin agonists. We have previously studied similar α-adrenoceptor responses. Identical rank orders of vasopressin agonists' potency were found for IP accumulation and contraction. The vasopressin analogue [1-(β-mercapto-β,β-cyclopentamethylene proprionic acid)-2-(O-methyl)tyrosine,D-arginine8] vasopressin, was shown to be a specific, reversible antagonist for both IP accumulation and contraction by all vasopressin agonists tested. No antagonism of vasopressin induced increases in IP accumulation or contraction were found using phenoxybenzamine. Therefore, (i) in this tissue vasopressin receptors mediate both contraction and IP accumulation; and (ii) vasopressin mediated responses appear to be direct effects not mediated via the activation of α-adrenoceptors. Demonstration of two entirely different receptors, mediating the same functional response, and which both promote IP production, is consistent with a general obligatory role for phosphoinositide catabolism in receptor mediated vascular smooth muscle contraction.",
author = "Fox, {Anthony W.} and Friedman, {Paul A.} and Abel, {Peter W.}",
year = "1987",
month = "3",
day = "3",
doi = "10.1016/0014-2999(87)90751-5",
language = "English",
volume = "135",
pages = "1--10",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Vasopressin receptor mediated contraction and [3H]inositol metabolism in rat tail artery

AU - Fox, Anthony W.

AU - Friedman, Paul A.

AU - Abel, Peter W.

PY - 1987/3/3

Y1 - 1987/3/3

N2 - Inositol phosphates (IP) production and contraction in isolated but otherwise intact rat tail artery were measured in response to stimulation by vasopressin agonists. We have previously studied similar α-adrenoceptor responses. Identical rank orders of vasopressin agonists' potency were found for IP accumulation and contraction. The vasopressin analogue [1-(β-mercapto-β,β-cyclopentamethylene proprionic acid)-2-(O-methyl)tyrosine,D-arginine8] vasopressin, was shown to be a specific, reversible antagonist for both IP accumulation and contraction by all vasopressin agonists tested. No antagonism of vasopressin induced increases in IP accumulation or contraction were found using phenoxybenzamine. Therefore, (i) in this tissue vasopressin receptors mediate both contraction and IP accumulation; and (ii) vasopressin mediated responses appear to be direct effects not mediated via the activation of α-adrenoceptors. Demonstration of two entirely different receptors, mediating the same functional response, and which both promote IP production, is consistent with a general obligatory role for phosphoinositide catabolism in receptor mediated vascular smooth muscle contraction.

AB - Inositol phosphates (IP) production and contraction in isolated but otherwise intact rat tail artery were measured in response to stimulation by vasopressin agonists. We have previously studied similar α-adrenoceptor responses. Identical rank orders of vasopressin agonists' potency were found for IP accumulation and contraction. The vasopressin analogue [1-(β-mercapto-β,β-cyclopentamethylene proprionic acid)-2-(O-methyl)tyrosine,D-arginine8] vasopressin, was shown to be a specific, reversible antagonist for both IP accumulation and contraction by all vasopressin agonists tested. No antagonism of vasopressin induced increases in IP accumulation or contraction were found using phenoxybenzamine. Therefore, (i) in this tissue vasopressin receptors mediate both contraction and IP accumulation; and (ii) vasopressin mediated responses appear to be direct effects not mediated via the activation of α-adrenoceptors. Demonstration of two entirely different receptors, mediating the same functional response, and which both promote IP production, is consistent with a general obligatory role for phosphoinositide catabolism in receptor mediated vascular smooth muscle contraction.

UR - http://www.scopus.com/inward/record.url?scp=0023136228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023136228&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(87)90751-5

DO - 10.1016/0014-2999(87)90751-5

M3 - Article

C2 - 2952516

AN - SCOPUS:0023136228

VL - 135

SP - 1

EP - 10

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -