V(D)J recombination: Mechanisms of initiation

David G. Schatz, Patrick C. Swanson

Research output: Contribution to journalArticle

263 Scopus citations

Abstract

V(D)J recombination assembles immunoglobulin and T cell receptor genes during lymphocyte development through a series of carefully orchestrated DNA breakage and rejoining events. DNA cleavage requires a series of protein-DNA complexes containing the RAG1 and RAG2 proteins and recombination signals that flank the recombining gene segments. In this review, we discuss recent advances in our understanding of the function and domain organization of the RAG proteins, the composition and structure of RAG-DNA complexes, and the pathways that lead to the formation of these complexes. We also consider the functional significance of RAG-mediated histone recognition and ubiquitin ligase activities, and the role played by RAG in ensuring proper repair of DNA breaks made during V(D)J recombination. Finally, we propose a model for the formation of RAG-DNA complexes that involves anchoring of RAG1 at the recombination signal nonamer and RAG2-dependent surveillance of adjoining DNA for suitable spacer and heptamer sequences.

Original languageEnglish (US)
Pages (from-to)167-202
Number of pages36
JournalAnnual Review of Genetics
Volume45
DOIs
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'V(D)J recombination: Mechanisms of initiation'. Together they form a unique fingerprint.

Cite this