Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry

Seksiri Arttamangkul, Andrew Plazek, Emily J. Platt, Haihong Jin, Thomas F. Murray, William T. Birdsong, Kenner C. Rice, David L. Farrens, John T. Williams

Research output: Contribution to journalArticle

Abstract

Identifying neurons that have functional opioid receptors is fundamental for the understanding of the cellular, synaptic and systems actions of opioids. Current techniques are limited to post hoc analyses of fixed tissues. Here we developed a fluorescent probe, naltrexamine-acylimidazole (NAI), to label opioid receptors based on a chemical approach termed 'traceless affinity labeling'. In this approach, a high affinity antagonist naltrexamine is used as the guide molecule for a transferring reaction of acylimidazole at the receptor. This reaction generates a fluorescent dye covalently linked to the receptor while naltrexamine is liberated and leaves the binding site. The labeling induced by this reagent allowed visualization of opioid-sensitive neurons in rat and mouse brains without loss of function of the fluorescently labeled receptors. The ability to locate endogenous receptors in living tissues will aid considerably in establishing the distribution and physiological role of opioid receptors in the CNS of wild type animals.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Oct 7 2019
Externally publishedYes

Fingerprint

Opioid Receptors
Neurons
Ligands
Fluorescent Dyes
Labeling
Opioid Analgesics
Living Wills
Tissue
Wild Animals
Rats
Labels
Brain
Animals
Visualization
Binding Sites
Molecules

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Arttamangkul, S., Plazek, A., Platt, E. J., Jin, H., Murray, T. F., Birdsong, W. T., ... Williams, J. T. (2019). Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. eLife, 8. https://doi.org/10.7554/eLife.49319

Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. / Arttamangkul, Seksiri; Plazek, Andrew; Platt, Emily J.; Jin, Haihong; Murray, Thomas F.; Birdsong, William T.; Rice, Kenner C.; Farrens, David L.; Williams, John T.

In: eLife, Vol. 8, 07.10.2019.

Research output: Contribution to journalArticle

Arttamangkul, S, Plazek, A, Platt, EJ, Jin, H, Murray, TF, Birdsong, WT, Rice, KC, Farrens, DL & Williams, JT 2019, 'Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry', eLife, vol. 8. https://doi.org/10.7554/eLife.49319
Arttamangkul, Seksiri ; Plazek, Andrew ; Platt, Emily J. ; Jin, Haihong ; Murray, Thomas F. ; Birdsong, William T. ; Rice, Kenner C. ; Farrens, David L. ; Williams, John T. / Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. In: eLife. 2019 ; Vol. 8.
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