Vitamin D supplementation reduces intimal hyperplasia and restenosis following coronary intervention in atherosclerotic swine

Gaurav K. Gupta, Tanupriya Agrawal, Vikrant Rai, Michael Del Core, William J. Hunter, Devendra K. Agrawal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vitamin D is a fat-soluble steroid hormone that activates vitamin D receptor to regulate multiple downstream signaling pathways and transcription of various target genes. There is an association between vitamin D deficiency and increased risk for cardiovascular disease. However, most of the studies are observational and associative in nature with limited data on clinical application. Thus, there is a need for more prospective randomized controlled studies to determine whether or not vitamin D supplementation provides cardiovascular protection. In this study, we examined the effects of the deficiency and supplementation of vitamin D on coronary restenosis following coronary intervention in atherosclerotic Yucatan microswine. Twelve Yucatan microswine were fed vitamin D-deficient (n = 4) or-sufficient (n = 8) high cholesterol diet for 6-months followed by coronary intervention. Post-intervention, swine in the vitamin D-sufficient high cholesterol diet group received daily oral supplementation of either 1,000 IU (n = 4) or 3,000 IU (n = 4) vitamin D3. Six months later, optical coherence tomography (OCT) was performed to monitor the development of intimal hyperplasia and restenosis. Animals were euthanized to isolate arteries for histomorphometric and immunohistochemical studies. Animals had graded levels of serum 25(OH)D; vitamin D-deficient (15.33 ± 1.45 ng/ml), vitamin D-sufficient + 1,000 IU oral vitamin D post-intervention (32.27 ± 1.20 ng/ml), and vitamin D-sufficient + 3,000 IU oral vitamin D post-intervention (51.00 ± 3.47 ng/ml). Findings from the OCT and histomorphometric studies showed a decrease in intimal hyperplasia and restenosis in vitamin D-supplemented compared to vitamin D-deficient swine. Vitamin D supplementation significantly decreased serum levels of TNF-? and IFN-?, upregulated serum levels of IL-10, and had no effect on serum IL-6 levels. These findings suggest that vitamin D supplementation limits neointimal formation following coronary intervention in atherosclerotic swine and provide the support for vitamin Dsupplementation to protect against the development of coronary restenosis.

Original languageEnglish
Article numbere0156857
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Coronary Restenosis
Tunica Intima
vitamin D
hyperplasia
Vitamin D
Hyperplasia
Swine
swine
Vitamin D Deficiency
Cholecalciferol
Optical Coherence Tomography
mouth
Serum
cholecalciferol
Optical tomography
tomography
high fat diet
Nutrition
Cholesterol
Animals

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Vitamin D supplementation reduces intimal hyperplasia and restenosis following coronary intervention in atherosclerotic swine. / Gupta, Gaurav K.; Agrawal, Tanupriya; Rai, Vikrant; Del Core, Michael; Hunter, William J.; Agrawal, Devendra K.

In: PLoS One, Vol. 11, No. 6, e0156857, 01.06.2016.

Research output: Contribution to journalArticle

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